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. Author manuscript; available in PMC: 2013 Jan 17.
Published in final edited form as: Biochemistry. 2012 Jan 3;51(2):597–607. doi: 10.1021/bi201221j

Figure 1.

Figure 1

Overlay of wild type (yellow) and Y54F (blue) streptavidin structures. The Y54F mutation has no significant effect on the overall protein backbone structure (left) or on the structure surrounding biotin and the mutation site (right).