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. 1994 Dec 11;22(24):5223–5228. doi: 10.1093/nar/22.24.5223

Splicing factor SF3a60 is the mammalian homologue of PRP9 of S.cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo.

A Krämer 1, P Legrain 1, F Mulhauser 1, K Gröning 1, R Brosi 1, G Bilbe 1
PMCID: PMC332064  PMID: 7816610

Abstract

A cDNA encoding the 60 kDa subunit of mammalian splicing factor SF3a has been isolated. The deduced protein sequence reveals a 30% identity to the PRP9 splicing protein of the yeast S.cerevisiae. The highest homology is present in a zinc finger-like region in the C-terminal domain of both proteins. The PRP9 zinc finger-like motif has been replaced by the equivalent region of mammalian SF3a60. The chimeric protein rescues the temperature-sensitive phenotype of the prp9-1 mutant strain demonstrating that not only the structure but also the function of this domain has been conserved during evolution.

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Selected References

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