Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1994 Dec 11;22(24):5265–5270. doi: 10.1093/nar/22.24.5265

Re-replication of SV40 minichromosomes is inhibited at the stage of chain elongation.

T Krude 1, A Winter 1, R Knippers 1
PMCID: PMC332070  PMID: 7816615

Abstract

The template activities of protein-free SV40 DNA and SV40 minichromosomes for DNA re-replication are compared in in vitro replication assays. Density substitution experiments and two-dimensional gel electrophoresis show that protein-free DNA can replicate for at least two cycles whereas salt-treated minichromosomes replicate only once. Re-replication of minichromosomes is blocked at the stage of replicative chain elongation suggesting that replicatively assembled chromatin has structural features that prevent a second round of replication.

Full text

PDF
5265

Images in this article

5266Image
on p.5266
5268Image
on p.5268
5268Image
on p.5268

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Almouzni G., Wolffe A. P. Replication-coupled chromatin assembly is required for the repression of basal transcription in vivo. Genes Dev. 1993 Oct;7(10):2033–2047. doi: 10.1101/gad.7.10.2033. [DOI] [PubMed] [Google Scholar]
  2. Bresnick E. H., John S., Berard D. S., LeFebvre P., Hager G. L. Glucocorticoid receptor-dependent disruption of a specific nucleosome on the mouse mammary tumor virus promoter is prevented by sodium butyrate. Proc Natl Acad Sci U S A. 1990 May;87(10):3977–3981. doi: 10.1073/pnas.87.10.3977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CAIRNS J. The bacterial chromosome and its manner of replication as seen by autoradiography. J Mol Biol. 1963 Mar;6:208–213. doi: 10.1016/s0022-2836(63)80070-4. [DOI] [PubMed] [Google Scholar]
  4. Challberg M. D., Kelly T. J. Animal virus DNA replication. Annu Rev Biochem. 1989;58:671–717. doi: 10.1146/annurev.bi.58.070189.003323. [DOI] [PubMed] [Google Scholar]
  5. Friedrich T. D., Laffin J., Lehman J. M. Simian virus 40 large T-antigen function is required for induction of tetraploid DNA content during lytic infection. J Virol. 1992 Jul;66(7):4576–4579. doi: 10.1128/jvi.66.7.4576-4579.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Germond J. E., Hirt B., Oudet P., Gross-Bellark M., Chambon P. Folding of the DNA double helix in chromatin-like structures from simian virus 40. Proc Natl Acad Sci U S A. 1975 May;72(5):1843–1847. doi: 10.1073/pnas.72.5.1843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gruss C., Gutierrez C., Burhans W. C., DePamphilis M. L., Koller T., Sogo J. M. Nucleosome assembly in mammalian cell extracts before and after DNA replication. EMBO J. 1990 Sep;9(9):2911–2922. doi: 10.1002/j.1460-2075.1990.tb07482.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gruss C., Wu J., Koller T., Sogo J. M. Disruption of the nucleosomes at the replication fork. EMBO J. 1993 Dec;12(12):4533–4545. doi: 10.1002/j.1460-2075.1993.tb06142.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hansen J. C., Wolffe A. P. Influence of chromatin folding on transcription initiation and elongation by RNA polymerase III. Biochemistry. 1992 Sep 1;31(34):7977–7988. doi: 10.1021/bi00149a032. [DOI] [PubMed] [Google Scholar]
  10. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  11. Hurwitz J., Dean F. B., Kwong A. D., Lee S. H. The in vitro replication of DNA containing the SV40 origin. J Biol Chem. 1990 Oct 25;265(30):18043–18046. [PubMed] [Google Scholar]
  12. Krude T., Knippers R. Minichromosome replication in vitro: inhibition of re-replication by replicatively assembled nucleosomes. J Biol Chem. 1994 Aug 19;269(33):21021–21029. [PubMed] [Google Scholar]
  13. Krude T., Knippers R. Nucleosome assembly during complementary DNA strand synthesis in extracts from mammalian cells. J Biol Chem. 1993 Jul 5;268(19):14432–14442. [PubMed] [Google Scholar]
  14. Krude T., Knippers R. Replication of SV40 minichromosomes in vitro. Chromosoma. 1992;102(1 Suppl):S83–S92. doi: 10.1007/BF02451790. [DOI] [PubMed] [Google Scholar]
  15. Krude T., Knippers R. Transfer of nucleosomes from parental to replicated chromatin. Mol Cell Biol. 1991 Dec;11(12):6257–6267. doi: 10.1128/mcb.11.12.6257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Krude T., de Maddalena C., Knippers R. A nucleosome assembly factor is a constituent of simian virus 40 minichromosomes. Mol Cell Biol. 1993 Feb;13(2):1059–1068. doi: 10.1128/mcb.13.2.1059. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lee D. Y., Hayes J. J., Pruss D., Wolffe A. P. A positive role for histone acetylation in transcription factor access to nucleosomal DNA. Cell. 1993 Jan 15;72(1):73–84. doi: 10.1016/0092-8674(93)90051-q. [DOI] [PubMed] [Google Scholar]
  18. Lehman J. M., Laffin J., Jacobberger J. W., Fogleman D. Analysis of simian virus 40 infection of CV-1 cells by quantitative two-color fluorescence with flow cytometry. Cytometry. 1988 Jan;9(1):52–59. doi: 10.1002/cyto.990090109. [DOI] [PubMed] [Google Scholar]
  19. Li J. J., Kelly T. J. Simian virus 40 DNA replication in vitro: specificity of initiation and evidence for bidirectional replication. Mol Cell Biol. 1985 Jun;5(6):1238–1246. doi: 10.1128/mcb.5.6.1238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Müller U., Zentgraf H., Eicken I., Keller W. Higher order structure of simian virus 40 chromatin. Science. 1978 Aug 4;201(4354):406–415. doi: 10.1126/science.208155. [DOI] [PubMed] [Google Scholar]
  21. Peck L. J., Wang J. C. Energetics of B-to-Z transition in DNA. Proc Natl Acad Sci U S A. 1983 Oct;80(20):6206–6210. doi: 10.1073/pnas.80.20.6206. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Perry C. A., Allis C. D., Annunziato A. T. Parental nucleosomes segregated to newly replicated chromatin are underacetylated relative to those assembled de novo. Biochemistry. 1993 Dec 14;32(49):13615–13623. doi: 10.1021/bi00212a029. [DOI] [PubMed] [Google Scholar]
  23. Perry C. A., Dadd C. A., Allis C. D., Annunziato A. T. Analysis of nucleosome assembly and histone exchange using antibodies specific for acetylated H4. Biochemistry. 1993 Dec 14;32(49):13605–13614. doi: 10.1021/bi00212a028. [DOI] [PubMed] [Google Scholar]
  24. Randall S. K., Kelly T. J. The fate of parental nucleosomes during SV40 DNA replication. J Biol Chem. 1992 Jul 15;267(20):14259–14265. [PubMed] [Google Scholar]
  25. Simanis V., Lane D. P. An immunoaffinity purification procedure for SV40 large T antigen. Virology. 1985 Jul 15;144(1):88–100. doi: 10.1016/0042-6822(85)90308-3. [DOI] [PubMed] [Google Scholar]
  26. Smith S., Stillman B. Purification and characterization of CAF-I, a human cell factor required for chromatin assembly during DNA replication in vitro. Cell. 1989 Jul 14;58(1):15–25. doi: 10.1016/0092-8674(89)90398-x. [DOI] [PubMed] [Google Scholar]
  27. Smith S., Stillman B. Stepwise assembly of chromatin during DNA replication in vitro. EMBO J. 1991 Apr;10(4):971–980. doi: 10.1002/j.1460-2075.1991.tb08031.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Snapka R. M. Topoisomerase inhibitors can selectively interfere with different stages of simian virus 40 DNA replication. Mol Cell Biol. 1986 Dec;6(12):4221–4227. doi: 10.1128/mcb.6.12.4221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Sogo J. M., Stahl H., Koller T., Knippers R. Structure of replicating simian virus 40 minichromosomes. The replication fork, core histone segregation and terminal structures. J Mol Biol. 1986 May 5;189(1):189–204. doi: 10.1016/0022-2836(86)90390-6. [DOI] [PubMed] [Google Scholar]
  30. Stillman B. W., Gluzman Y. Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells. Mol Cell Biol. 1985 Aug;5(8):2051–2060. doi: 10.1128/mcb.5.8.2051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Stillman B. Initiation of eukaryotic DNA replication in vitro. Annu Rev Cell Biol. 1989;5:197–245. doi: 10.1146/annurev.cb.05.110189.001213. [DOI] [PubMed] [Google Scholar]
  32. Sugasawa K., Ishimi Y., Eki T., Hurwitz J., Kikuchi A., Hanaoka F. Nonconservative segregation of parental nucleosomes during simian virus 40 chromosome replication in vitro. Proc Natl Acad Sci U S A. 1992 Feb 1;89(3):1055–1059. doi: 10.1073/pnas.89.3.1055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Sugasawa K., Murakami Y., Miyamoto N., Hanaoka F., Ui M. Assembly of nascent DNA into nucleosome structures in simian virus 40 chromosomes by HeLa cell extract. J Virol. 1990 Oct;64(10):4820–4829. doi: 10.1128/jvi.64.10.4820-4829.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Sundin O., Varshavsky A. Arrest of segregation leads to accumulation of highly intertwined catenated dimers: dissection of the final stages of SV40 DNA replication. Cell. 1981 Sep;25(3):659–669. doi: 10.1016/0092-8674(81)90173-2. [DOI] [PubMed] [Google Scholar]
  35. Wobbe C. R., Dean F., Weissbach L., Hurwitz J. In vitro replication of duplex circular DNA containing the simian virus 40 DNA origin site. Proc Natl Acad Sci U S A. 1985 Sep;82(17):5710–5714. doi: 10.1073/pnas.82.17.5710. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES