Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1990 Sep 25;18(18):5375–5380. doi: 10.1093/nar/18.18.5375

Evidence for a feedback regulated back-up promoter which controls permanent expression of a Dictyostelium gene.

M Maniak 1, W Nellen 1
PMCID: PMC332212  PMID: 2216709

Abstract

We have previously described the elements of two overlapping promoters regulating differential expression of the Dictyostelium P8A7 gene. One promoter was constitutively active but stimulated during development (S-promoter), while the other (L-promoter) was found to be induced by a variety of stimuli. This effect was mediated by the induction (i-) element. By a series of antisense transformation experiments we here provide evidence that this element is the target of a feedback control circuit. In wild type cells, feedback regulation activates the L-promoter in unfavourable environmental situations when transcription from the S-promoter is insufficient to generate adequate amounts of protein. A model for the function of the feedback loop under natural and experimental conditions is presented.

Full text

PDF
5375

Images in this article

5376Image
on p.5376
5377Image
on p.5377
5378Image
on p.5378
5378Image
on p.5378
5378Image
on p.5378

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akerblom I. E., Slater E. P., Beato M., Baxter J. D., Mellon P. L. Negative regulation by glucocorticoids through interference with a cAMP responsive enhancer. Science. 1988 Jul 15;241(4863):350–353. doi: 10.1126/science.2838908. [DOI] [PubMed] [Google Scholar]
  2. Bass B. L., Weintraub H. A developmentally regulated activity that unwinds RNA duplexes. Cell. 1987 Feb 27;48(4):607–613. doi: 10.1016/0092-8674(87)90239-x. [DOI] [PubMed] [Google Scholar]
  3. Cappello J., Zuker C., Lodish H. F. Repetitive Dictyostelium heat-shock promotor functions in Saccharomyces cerevisiae. Mol Cell Biol. 1984 Apr;4(4):591–598. doi: 10.1128/mcb.4.4.591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Corbin V., Maniatis T. Role of transcriptional interference in the Drosophila melanogaster Adh promoter switch. Nature. 1989 Jan 19;337(6204):279–282. doi: 10.1038/337279a0. [DOI] [PubMed] [Google Scholar]
  5. Corney A. J., Richards A. J., Phillpots T., Hames B. D. Developmental regulation of cell-type-enriched mRNAs in Dictyostelium discoideum. Mol Microbiol. 1990 Apr;4(4):613–623. doi: 10.1111/j.1365-2958.1990.tb00630.x. [DOI] [PubMed] [Google Scholar]
  6. Crowley T. E., Nellen W., Gomer R. H., Firtel R. A. Phenocopy of discoidin I-minus mutants by antisense transformation in Dictyostelium. Cell. 1985 Dec;43(3 Pt 2):633–641. doi: 10.1016/0092-8674(85)90235-1. [DOI] [PubMed] [Google Scholar]
  7. Faure M., Franke J., Hall A. L., Podgorski G. J., Kessin R. H. The cyclic nucleotide phosphodiesterase gene of Dictyostelium discoideum contains three promoters specific for growth, aggregation, and late development. Mol Cell Biol. 1990 May;10(5):1921–1930. doi: 10.1128/mcb.10.5.1921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gerisch G. Cyclic AMP and other signals controlling cell development and differentiation in Dictyostelium. Annu Rev Biochem. 1987;56:853–879. doi: 10.1146/annurev.bi.56.070187.004225. [DOI] [PubMed] [Google Scholar]
  9. Gerisch G. Inter-relation of cell adhesion and differentiation in Dictyostelium discoideum. J Cell Sci Suppl. 1986;4:201–219. doi: 10.1242/jcs.1986.supplement_4.13. [DOI] [PubMed] [Google Scholar]
  10. Howard P. K., Ahern K. G., Firtel R. A. Establishment of a transient expression system for Dictyostelium discoideum. Nucleic Acids Res. 1988 Mar 25;16(6):2613–2623. doi: 10.1093/nar/16.6.2613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Knecht D. A., Loomis W. F. Antisense RNA inactivation of myosin heavy chain gene expression in Dictyostelium discoideum. Science. 1987 May 29;236(4805):1081–1086. doi: 10.1126/science.3576221. [DOI] [PubMed] [Google Scholar]
  12. Maniak M., Nellen W. A developmentally regulated membrane protein gene in Dictyostelium discoideum is also induced by heat shock and cold shock. Mol Cell Biol. 1988 Jan;8(1):153–159. doi: 10.1128/mcb.8.1.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Maniak M., Nellen W. Two separable promoters control different aspects of expression of a Dictyostelium gene. Nucleic Acids Res. 1990 Jun 11;18(11):3211–3217. doi: 10.1093/nar/18.11.3211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Maniak M., Saur U., Nellen W. A colony-blot technique for the detection of specific transcripts in eukaryotes. Anal Biochem. 1989 Jan;176(1):78–81. doi: 10.1016/0003-2697(89)90275-3. [DOI] [PubMed] [Google Scholar]
  15. Mann S. K., Firtel R. A. Two-phase regulatory pathway controls cAMP receptor-mediated expression of early genes in Dictyostelium. Proc Natl Acad Sci U S A. 1989 Mar;86(6):1924–1928. doi: 10.1073/pnas.86.6.1924. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. May T., Kern H., Müller-Taubenberger A., Nellen W. Identification of a cis-acting element controlling induction of early gene expression in Dictyostelium discoideum. Mol Cell Biol. 1989 Nov;9(11):4653–4659. doi: 10.1128/mcb.9.11.4653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Morris H. R., Taylor G. W., Masento M. S., Jermyn K. A., Kay R. R. Chemical structure of the morphogen differentiation inducing factor from Dictyostelium discoideum. 1987 Aug 27-Sep 2Nature. 328(6133):811–814. doi: 10.1038/328811a0. [DOI] [PubMed] [Google Scholar]
  18. Müller-Taubenberger A., Hagmann J., Noegel A., Gerisch G. Ubiquitin gene expression in Dictyostelium is induced by heat and cold shock, cadmium, and inhibitors of protein synthesis. J Cell Sci. 1988 May;90(Pt 1):51–58. doi: 10.1242/jcs.90.1.51. [DOI] [PubMed] [Google Scholar]
  19. Nellen W., Saur U. Cell-cycle dependent transformation competence in Dictyostelium discoideum. Biochem Biophys Res Commun. 1988 Jul 15;154(1):54–59. doi: 10.1016/0006-291x(88)90648-1. [DOI] [PubMed] [Google Scholar]
  20. Parag H. A., Raboy B., Kulka R. G. Effect of heat shock on protein degradation in mammalian cells: involvement of the ubiquitin system. EMBO J. 1987 Jan;6(1):55–61. doi: 10.1002/j.1460-2075.1987.tb04718.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Proudfoot N. J. Transcriptional interference and termination between duplicated alpha-globin gene constructs suggests a novel mechanism for gene regulation. Nature. 1986 Aug 7;322(6079):562–565. doi: 10.1038/322562a0. [DOI] [PubMed] [Google Scholar]
  22. Schibler U., Sierra F. Alternative promoters in developmental gene expression. Annu Rev Genet. 1987;21:237–257. doi: 10.1146/annurev.ge.21.120187.001321. [DOI] [PubMed] [Google Scholar]
  23. Serfling E. Autoregulation--a common property of eukaryotic transcription factors? Trends Genet. 1989 May;5(5):131–133. doi: 10.1016/0168-9525(89)90049-8. [DOI] [PubMed] [Google Scholar]
  24. Theodorakis N. G., Zand D. J., Kotzbauer P. T., Williams G. T., Morimoto R. I. Hemin-induced transcriptional activation of the HSP70 gene during erythroid maturation in K562 cells is due to a heat shock factor-mediated stress response. Mol Cell Biol. 1989 Aug;9(8):3166–3173. doi: 10.1128/mcb.9.8.3166. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Witke W., Schleicher M., Lottspeich F., Noegel A. Studies on the transcription, translation, and structure of alpha-actinin in Dictyostelium discoideum. J Cell Biol. 1986 Sep;103(3):969–975. doi: 10.1083/jcb.103.3.969. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES