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. 1990 Oct 11;18(19):5871–5877. doi: 10.1093/nar/18.19.5871

Characterization of a novel plasmid-like element in Neurospora crassa derived mostly from the mitochondrial DNA.

A Almasan 1, N C Mishra 1
PMCID: PMC332327  PMID: 2145549

Abstract

We have identified a plasmid-like element within mitochondria of Neurospora crassa strain stp-B1. It is derived from the EcoRI-4 and EcoRI-6 regions of the mitochondrial DNA, and an additional 124 bp DNA segment of unknown origin. The plasmid DNA consists of an oligomeric series of circular molecules of monomer length 2.2 kbp. The abundance of the plasmid suggests its autonomous replication and the presence of an efficient origin of replication. An unusually large number of palindromes capable of forming secondary structures are present in the plasmid. Such a palindrome, located near sequences reminiscent of mammalian and fungal mtDNA origins of replication, may define the replication origin of the plasmid. This putative origin might also represent the replication origin of the wild-type mtDNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agsteribbe E., Hartog M. Processing of precursor RNAs from mitochondria of Neurospora crassa. Nucleic Acids Res. 1987 Sep 25;15(18):7249–7263. doi: 10.1093/nar/15.18.7249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Agsteribbe E., Hartog M., de Vries H. Duplication of the tRNA(MMet) and tRNA(Cys) genes and of fragments of a gene encoding a subunit of the NADH dehydrogenase complex in Neurospora grassa mitochondrial DNA. Curr Genet. 1989 Jan;15(1):57–62. doi: 10.1007/BF00445752. [DOI] [PubMed] [Google Scholar]
  3. Agsteribbe E., Kroon A. M., van Bruggen E. F. Circular DNA from mitochondria of Neurospora crassa. Biochim Biophys Acta. 1972 May 10;269(2):299–303. doi: 10.1016/0005-2787(72)90439-x. [DOI] [PubMed] [Google Scholar]
  4. Akins R. A., Kelley R. L., Lambowitz A. M. Mitochondrial plasmids of Neurospora: integration into mitochondrial DNA and evidence for reverse transcription in mitochondria. Cell. 1986 Nov 21;47(4):505–516. doi: 10.1016/0092-8674(86)90615-x. [DOI] [PubMed] [Google Scholar]
  5. Almasan A., Mishra N. C. Molecular characterization of the mitochondrial DNA of a new stopper mutant ER-3 of Neurospora crassa. Genetics. 1988 Dec;120(4):935–945. doi: 10.1093/genetics/120.4.935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bertrand H., Collins R. A., Stohl L. L., Goewert R. R., Lambowitz A. M. Deletion mutants of Neurospora crassa mitochondrial DNA and their relationship to the "stop-start" growth phenotype. Proc Natl Acad Sci U S A. 1980 Oct;77(10):6032–6036. doi: 10.1073/pnas.77.10.6032. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Biggin M. D., Gibson T. J., Hong G. F. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963–3965. doi: 10.1073/pnas.80.13.3963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Brock N. Oxazaphosphorine cytostatics: past-present-future. Seventh Cain Memorial Award lecture. Cancer Res. 1989 Jan 1;49(1):1–7. [PubMed] [Google Scholar]
  9. Brown G. G., Gadaleta G., Pepe G., Saccone C., Sbisà E. Structural conservation and variation in the D-loop-containing region of vertebrate mitochondrial DNA. J Mol Biol. 1986 Dec 5;192(3):503–511. doi: 10.1016/0022-2836(86)90272-x. [DOI] [PubMed] [Google Scholar]
  10. Carroll S. M., DeRose M. L., Gaudray P., Moore C. M., Needham-Vandevanter D. R., Von Hoff D. D., Wahl G. M. Double minute chromosomes can be produced from precursors derived from a chromosomal deletion. Mol Cell Biol. 1988 Apr;8(4):1525–1533. doi: 10.1128/mcb.8.4.1525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Chen E. Y., Seeburg P. H. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA. 1985 Apr;4(2):165–170. doi: 10.1089/dna.1985.4.165. [DOI] [PubMed] [Google Scholar]
  12. Clayton D. A. Replication of animal mitochondrial DNA. Cell. 1982 Apr;28(4):693–705. doi: 10.1016/0092-8674(82)90049-6. [DOI] [PubMed] [Google Scholar]
  13. Collins R. A., Stohl L. L., Cole M. D., Lambowitz A. M. Characterization of a novel plasmid DNA found in mitochondria of N. crassa. Cell. 1981 May;24(2):443–452. doi: 10.1016/0092-8674(81)90335-4. [DOI] [PubMed] [Google Scholar]
  14. Cummings D. J., Belcour L., Grandchamp C. Mitochondrial DNA from Podospora anserina. I. Isolation and characterization. Mol Gen Genet. 1979 Mar 27;171(3):229–238. doi: 10.1007/BF00267577. [DOI] [PubMed] [Google Scholar]
  15. Dente L., Cesareni G., Cortese R. pEMBL: a new family of single stranded plasmids. Nucleic Acids Res. 1983 Mar 25;11(6):1645–1655. doi: 10.1093/nar/11.6.1645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Fangman W. L., Henly J. W., Churchill G., Brewer B. J. Stable maintenance of a 35-base-pair yeast mitochondrial genome. Mol Cell Biol. 1989 May;9(5):1917–1921. doi: 10.1128/mcb.9.5.1917. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Gross S. R., Levine P. H., Metzger S., Glaser G. Recombination and replication of plasmid-like derivatives of a short section of the mitochondrial chromosome of Neurospora crassa. Genetics. 1989 Apr;121(4):693–701. doi: 10.1093/genetics/121.4.693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Gross S. R., Mary A., Levine P. H. Change in chromosome number associated with a double deletion in the Neurospora crassa mitochondrial chromosome. Genetics. 1989 Apr;121(4):685–691. doi: 10.1093/genetics/121.4.685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kennell J. C., Lambowitz A. M. Development of an in vitro transcription system for Neurospora crassa mitochondrial DNA and identification of transcription initiation sites. Mol Cell Biol. 1989 Sep;9(9):3603–3613. doi: 10.1128/mcb.9.9.3603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Kuiper M. T., Lambowitz A. M. A novel reverse transcriptase activity associated with mitochondrial plasmids of Neurospora. Cell. 1988 Nov 18;55(4):693–704. doi: 10.1016/0092-8674(88)90228-0. [DOI] [PubMed] [Google Scholar]
  22. Lambowitz A. M. Preparation and analysis of mitochondrial ribosomes. Methods Enzymol. 1979;59:421–433. doi: 10.1016/0076-6879(79)59103-4. [DOI] [PubMed] [Google Scholar]
  23. Lin J. J., Garber R. C., Yoder O. C. Nucleotide sequence of a fungal plasmid-like DNA containing the mitochondrial ATPase subunit 6 gene. Nucleic Acids Res. 1988 Oct 25;16(20):9875–9875. doi: 10.1093/nar/16.20.9875. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Macino G., Morelli G. Cytochrome oxidase subunit 2 gene in Neurospora crassa mitochondria. J Biol Chem. 1983 Nov 10;258(21):13230–13235. [PubMed] [Google Scholar]
  25. Mannella C. A., Goewert R. R., Lambowitz A. M. Characterization of variant Neurospora crassa mitochondrial DNAs which contain tandem reiterations. Cell. 1979 Dec;18(4):1197–1207. doi: 10.1016/0092-8674(79)90232-0. [DOI] [PubMed] [Google Scholar]
  26. Meinkoth J., Killary A. M., Fournier R. E., Wahl G. M. Unstable and stable CAD gene amplification: importance of flanking sequences and nuclear environment in gene amplification. Mol Cell Biol. 1987 Apr;7(4):1415–1424. doi: 10.1128/mcb.7.4.1415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Nargang F. E., Bell J. B., Stohl L. L., Lambowitz A. M. The DNA sequence and genetic organization of a Neurospora mitochondrial plasmid suggest a relationship to introns and mobile elements. Cell. 1984 Sep;38(2):441–453. doi: 10.1016/0092-8674(84)90499-9. [DOI] [PubMed] [Google Scholar]
  28. Natvig D. O., May G., Taylor J. W. Distribution and evolutionary significance of mitochondrial plasmids in Neurospora spp. J Bacteriol. 1984 Jul;159(1):288–293. doi: 10.1128/jb.159.1.288-293.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Stohl L. L., Collins R. A., Cole M. D., Lambowitz A. M. Characterization of two new plasmid DNAs found in mitochondria of wild-type Neurospora intermedia strains. Nucleic Acids Res. 1982 Mar 11;10(5):1439–1458. doi: 10.1093/nar/10.5.1439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Subia N. L., Kogoma T. Concatemer formation of ColE1-type plasmids in mutants of Escherichia coli lacking RNase H activity. J Mol Biol. 1986 Jun 5;189(3):389–399. doi: 10.1016/0022-2836(86)90311-6. [DOI] [PubMed] [Google Scholar]
  32. Tabor S., Richardson C. C. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767–4771. doi: 10.1073/pnas.84.14.4767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Turker M. S., Domenico J. M., Cummings D. J. A novel family of mitochondrial plasmids associated with longevity mutants of Podospora anserina. J Biol Chem. 1987 Feb 15;262(5):2250–2255. [PubMed] [Google Scholar]
  34. Yin S., Heckman J., RajBhandary U. L. Highly conserved GC-rich palindromic DNA sequences flank tRNA genes in Neurospora crassa mitochondria. Cell. 1981 Nov;26(3 Pt 1):325–332. doi: 10.1016/0092-8674(81)90201-4. [DOI] [PubMed] [Google Scholar]
  35. de Vries H., Alzner-DeWeerd B., Breitenberger C. A., Chang D. D., de Jonge J. C., RajBhandary U. L. The E35 stopper mutant of Neurospora crassa: precise localization of deletion endpoints in mitochondrial DNA and evidence that the deleted DNA codes for a subunit of NADH dehydrogenase. EMBO J. 1986 Apr;5(4):779–785. doi: 10.1002/j.1460-2075.1986.tb04281.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. de Zamaroczy M., Faugeron-Fonty G., Baldacci G., Goursot R., Bernardi G. The ori sequences of the mitochondrial genome of a wild-type yeast strain: number, location, orientation and structure. Gene. 1984 Dec;32(3):439–457. doi: 10.1016/0378-1119(84)90019-2. [DOI] [PubMed] [Google Scholar]

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