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. 2012 Jan 31;33(4):760–769. doi: 10.1093/carcin/bgs023

Fig. 5.

Fig. 5.

Knockdown of ETS2 or overexpression of miR-196b affected the morphological changes and actin organization of AGS cells. (A) Knockdown of ETS2 and overexpression of miR-196b in AGS cells were confirmed by reverse transcription–PCR. (B) The morphology of AGS cells after downregulation of ETS2 by shRNA or overexpression of miR-196b. Altered morphologies of AGS cells were revealed by phase contrast microscopy. Images were taken 5–7 days after infection. Original magnification: ×200. (C) Loss of actin organization in shETS2- and miR-196b-treated cells. Control shLuc-treated, shETS2- and miR-196b-treated AGS cells were fixed and stained with rhodamine–phalloidine and 4′,6-diamidino-2-phenylindole to detect F-actin and the nucleus, respectively. Bar, 20 μm.