Fig. 6.

Knockdown of ETS2 or overexpression of miR-196b resulted in induction of migration and invasion. ETS2 knockdown and miR-196b overexpression resulted in a 30–50% induction in migration (A) and invasion (B) activity in AGS cells. AGS cells were infected with control shLuc, shETS2 or miR-196b for 16 h followed by a 2 day puromycin (2 μg/ml) selection before the cells were plated for the migration and invasion assay. The cell migration and invasion abilities were assessed as described in the Materials and methods. A total of 35 fields were counted for every filter. The data are an average of triplicates for each condition. *P < 0.05, **P < 0.01.