Fig. 2.

Curcumin induction of ROS activates Erk1/2 and JNK in tumor cells. (A) Rh30 cells were treated with 0–40 μM curcumin for 24 h, (B) with 20 μM curcumin for 0–24 h or (C) pretreated with NAC (5 mM) for 1 h and then exposed to curcumin (0–20 μM) for 24 h. The lysates were subjected to western blot analysis using indicated antibodies. The blots were probed for β-tubulin for a loading control.