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. 1990 Nov 11;18(21):6347–6351. doi: 10.1093/nar/18.21.6347

B2 RNA and 7SK RNA, RNA polymerase III transcripts, have a cap-like structure at their 5' end.

G P Shumyatsky 1, S V Tillib 1, D A Kramerov 1
PMCID: PMC332502  PMID: 1700854

Abstract

We found that hydrolysates of poly(A)+ RNA from Ehrlich ascites carcinoma cells which were transcribed by RNA polymerase III contained an unusual component designated as X. It was part of B2 RNA representing a transcript of B2 retroposon, typical of rodents. The component X possesses a cap-like structure, xppp5'G, where x has a non-nucleotide structure. About half of all B2 RNAs contained this group at the 5' end. Previously, Epstein et al. (1) detected a similar structure at the 5' end of small nuclear U6 RNA. Later, Singh and Reddy (2) showed methyl to be the blocking group in the component x of U6 RNA. Besides B2 RNA, we found 5' ends containing methyl groups in 7SK RNA.

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Selected References

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