Figure 2.

Over-expression of VEGF₁₆₅b in mouse ovary reduces litter size. (A) Mouse ovaries from wild-type or transgenic MMTV-VEGF₁₆₅b mice as shown were taken and RNA was extracted. RT-PCR was performed using primers specific for the human transgene. (B) Mouse tissues were fixed and stained using an anti-human VEGF antibody that detects all human isoforms but does not detect mouse VEGF. (C) Measurement of litter size in 10 transgenic (TG) or wild-type (WT) females mated to wild-type males. **P<0.01 paired t-test, n=10 compared with littermate control. Wild-type females were also mated to TG males. NS, not significant compared with wild-type (unpaired t-test).