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. 1991 Dec;19(25):7185–7192. doi: 10.1093/nar/19.25.7185

Nuclease Bal-31 mapping of proteins bound to a tRNA(tyr) gene in SV40 minichromosomes.

S R Scanlon 1, W R Folk 1
PMCID: PMC332565  PMID: 1662809

Abstract

We have analyzed proteins bound to active and to inactive tRNA(tyr) genes imbedded in the late coding region of SV40 minichromosomal DNA. Bal-31 nuclease resection from the 5' and 3' sides of the active tRNA(tyr) gene reveals proteins bound to the 5' flank, to the promoter 'A' block, to an intragenic sequence, to the promoter 'B' block and to a 3' downstream terminator/pause sequence. The proteins bound near the promoter 'B' block and the downstream terminator/pause sequence are reduced or eliminated by an inactivating deletion in the tRNA(tyr) 'B block'. That proteins are detected in the 5' flank and over the promoter 'A block' of the inactive gene contrasts with current notions regarding the requirement for a functional 'B' block for binding of transcription factors.

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