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. 1990 Dec 11;18(23):6915–6919. doi: 10.1093/nar/18.23.6915

Complete sequence of the extrachromosomal rDNA molecule from the ciliate Tetrahymena thermophila strain B1868VII.

J Engberg 1, H Nielsen 1
PMCID: PMC332750  PMID: 2263454

Abstract

The recent development of rDNA vectors for transformation of Tetrahymena combined with improved microinjection technology should lead to a renewed interest in this organism. In particular, the rDNA itself constitutes an attractive system for biochemical studies. The rDNA is amplified to a level of 2% of the total DNA and exists as extrachromosomal molecules. Furthermore, the rDNA is homogeneous in sequence because it is derived from a single gene during sexual reorganization. In order to facilitate studies of this molecule, we report here a compilation of previously published sequence information together with new sequence data that completes the entire sequence of the 21 kb rDNA molecule.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonven B. J., Gocke E., Westergaard O. A high affinity topoisomerase I binding sequence is clustered at DNAase I hypersensitive sites in Tetrahymena R-chromatin. Cell. 1985 Jun;41(2):541–551. doi: 10.1016/s0092-8674(85)80027-1. [DOI] [PubMed] [Google Scholar]
  2. Brunk C. F., Navas P. Transformation of Tetrahymena thermophila by electroporation and parameters effecting cell survival. Exp Cell Res. 1988 Feb;174(2):525–532. doi: 10.1016/0014-4827(88)90322-9. [DOI] [PubMed] [Google Scholar]
  3. Cech T. R., Bass B. L. Biological catalysis by RNA. Annu Rev Biochem. 1986;55:599–629. doi: 10.1146/annurev.bi.55.070186.003123. [DOI] [PubMed] [Google Scholar]
  4. Cech T. R., Brehm S. L. Replication of the extrachromosomal ribosomal RNA genes of Tetrahymena thermophilia. Nucleic Acids Res. 1981 Jul 24;9(14):3531–3543. doi: 10.1093/nar/9.14.3531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Challoner P. B., Amin A. A., Pearlman R. E., Blackburn E. H. Conserved arrangements of repeated DNA sequences in nontranscribed spacers of ciliate ribosomal RNA genes: evidence for molecular coevolution. Nucleic Acids Res. 1985 Apr 11;13(7):2661–2680. doi: 10.1093/nar/13.7.2661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Colavito-Shepanski M., Gorovsky M. A. The histone content of Tetrahymena ribosomal gene-containing chromatin. J Biol Chem. 1983 May 10;258(9):5944–5954. [PubMed] [Google Scholar]
  7. Din N., Engberg J. Extrachromosomal ribosomal RNA genes in Tetrahymena: structure and evolution. J Mol Biol. 1979 Nov 5;134(3):555–574. doi: 10.1016/0022-2836(79)90367-x. [DOI] [PubMed] [Google Scholar]
  8. Din N., Engberg J., Gall J. G. The nucleotide sequence at the transcription termination site of the ribosomal RNA gene in Tetrahymena thermophila. Nucleic Acids Res. 1982 Mar 11;10(5):1503–1513. doi: 10.1093/nar/10.5.1503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Eckert W. A., Kaffenberger W., Krohne G., Franke W. W. Introduction of hidden breaks during rRNA maturation and ageing in Tetrahymena pyriformis. Eur J Biochem. 1978 Jul 3;87(3):607–616. doi: 10.1111/j.1432-1033.1978.tb12413.x. [DOI] [PubMed] [Google Scholar]
  10. Engberg J., Din N., Saiga H., Higashinakagawa T. Nucleotide sequence of the 5'-terminal coding region for pre-rRNA and mature 17S rRNA in Tetrahymena thermophila rDNA. Nucleic Acids Res. 1984 Jan 25;12(2):959–972. doi: 10.1093/nar/12.2.959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Engberg J., Nielsen H., Lenaers G., Murayama O., Fujitani H., Higashinakagawa T. Comparison of primary and secondary 26S rRNA structures in two Tetrahymena species: evidence for a strong evolutionary and structural constraint in expansion segments. J Mol Evol. 1990 Jun;30(6):514–521. doi: 10.1007/BF02101107. [DOI] [PubMed] [Google Scholar]
  12. Engberg J. Strong sequence conservation of a 38 bp region near the center of the extrachromosomal rDNA palindrome in different Tetrahymena species. Nucleic Acids Res. 1983 Jul 25;11(14):4939–4946. doi: 10.1093/nar/11.14.4939. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Engberg J. The ribosomal RNA genes of Tetrahymena: structure and function. Eur J Cell Biol. 1985 Jan;36(1):133–151. [PubMed] [Google Scholar]
  14. Greider C. W., Blackburn E. H. A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis. Nature. 1989 Jan 26;337(6205):331–337. doi: 10.1038/337331a0. [DOI] [PubMed] [Google Scholar]
  15. Greider C. W., Blackburn E. H. The telomere terminal transferase of Tetrahymena is a ribonucleoprotein enzyme with two kinds of primer specificity. Cell. 1987 Dec 24;51(6):887–898. doi: 10.1016/0092-8674(87)90576-9. [DOI] [PubMed] [Google Scholar]
  16. Kan N. C., Gall J. G. The intervening sequence of the ribosomal RNA gene is highly conserved between two Tetrahymena species. Nucleic Acids Res. 1982 May 11;10(9):2809–2822. doi: 10.1093/nar/10.9.2809. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kiss G. B., Amin A. A., Pearlman R. E. Two separate regions of the extrachromosomal ribosomal deoxyribonucleic acid of Tetrahymena thermophila enable autonomous replication of plasmids in Saccharomyces cerevisiae. Mol Cell Biol. 1981 Jun;1(6):535–543. doi: 10.1128/mcb.1.6.535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kiss G. B., Pearlman R. E. Extrachromosomal rDNA of Tetrahymena thermophila is not a perfect palindrome. Gene. 1981 Apr;13(3):281–287. doi: 10.1016/0378-1119(81)90032-9. [DOI] [PubMed] [Google Scholar]
  19. Kister K. P., Kaffenberger W., Eckert W. A. In vitro synthesis and processing of pre-rRNA in isolated macronuclei from Tetrahymena. Eur J Cell Biol. 1988 Jun;46(2):233–243. [PubMed] [Google Scholar]
  20. Larson D. D., Blackburn E. H., Yaeger P. C., Orias E. Control of rDNA replication in Tetrahymena involves a cis-acting upstream repeat of a promoter element. Cell. 1986 Oct 24;47(2):229–240. doi: 10.1016/0092-8674(86)90445-9. [DOI] [PubMed] [Google Scholar]
  21. Luehrsen K. R., Baum M. P., Orias E. A restriction fragment length polymorphism in the 5' non-transcribed spacer of the rDNA of Tetrahymena thermophila inbred strains B and C3. Gene. 1987;55(2-3):169–178. doi: 10.1016/0378-1119(87)90277-0. [DOI] [PubMed] [Google Scholar]
  22. Luehrsen K. R., Baum M. P., Orias E. A restriction fragment length polymorphism in the 5' non-transcribed spacer of the rDNA of Tetrahymena thermophila inbred strains B and C3. Gene. 1987;55(2-3):169–178. doi: 10.1016/0378-1119(87)90277-0. [DOI] [PubMed] [Google Scholar]
  23. Niles E. G. Identification of multiple sites in the promoter region of the Tetrahymena pyriformis rRNA gene which bind the Escherichia coli catabolite regulatory protein. J Biol Chem. 1985 Jan 10;260(1):672–678. [PubMed] [Google Scholar]
  24. Niles E. G., Sutiphong J., Haque S. Structure of the Tetrahymena pyriformis rRNA gene. Nucleotide sequence of the transcription initiation region. J Biol Chem. 1981 Dec 25;256(24):12849–12856. [PubMed] [Google Scholar]
  25. Pan W. C., Blackburn E. H. Single extrachromosomal ribosomal RNA gene copies are synthesized during amplification of the rDNA in Tetrahymena. Cell. 1981 Feb;23(2):459–466. doi: 10.1016/0092-8674(81)90141-0. [DOI] [PubMed] [Google Scholar]
  26. Sogin M. L., Ingold A., Karlok M., Nielsen H., Engberg J. Phylogenetic evidence for the acquisition of ribosomal RNA introns subsequent to the divergence of some of the major Tetrahymena groups. EMBO J. 1986 Dec 20;5(13):3625–3630. doi: 10.1002/j.1460-2075.1986.tb04691.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Spangler E. A., Blackburn E. H. The nucleotide sequence of the 17S ribosomal RNA gene of Tetrahymena thermophila and the identification of point mutations resulting in resistance to the antibiotics paromomycin and hygromycin. J Biol Chem. 1985 May 25;260(10):6334–6340. [PubMed] [Google Scholar]
  28. Sutiphong J., Matzura C., Niles E. G. Characterization of a crude selective PolI transcription system from Tetrahymena pyriformis. Biochemistry. 1984 Dec 18;23(26):6319–6326. doi: 10.1021/bi00321a005. [DOI] [PubMed] [Google Scholar]
  29. Sweeney R., Yao M. C. Identifying functional regions of rRNA by insertion mutagenesis and complete gene replacement in Tetrahymena thermophila. EMBO J. 1989 Mar;8(3):933–938. doi: 10.1002/j.1460-2075.1989.tb03454.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Tondravi M. M., Yao M. C. Transformation of Tetrahymena thermophila by microinjection of ribosomal RNA genes. Proc Natl Acad Sci U S A. 1986 Jun;83(12):4369–4373. doi: 10.1073/pnas.83.12.4369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Van Bell C. T. The 5S and 5.8S ribosomal RNA sequences of Tetrahymena thermophila and T. pyriformis. J Protozool. 1985 Nov;32(4):640–644. doi: 10.1111/j.1550-7408.1985.tb03093.x. [DOI] [PubMed] [Google Scholar]
  32. Yu G. L., Hasson M., Blackburn E. H. Circular ribosomal DNA plasmids transform Tetrahymena thermophila by homologous recombination with endogenous macronuclear ribosomal DNA. Proc Natl Acad Sci U S A. 1988 Jul;85(14):5151–5155. doi: 10.1073/pnas.85.14.5151. [DOI] [PMC free article] [PubMed] [Google Scholar]

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