Figure 4.

Patch clamp recording in α3β4-containing HEK cells. Sample trace of the blocking effect of 10 nM AT-1001 against 10 nM Epibatidine activation (66% block, n=3). Whole-cell voltage-clamp recordings were made using Axon 700A and PClamp 8.2. Experiments were conducted within 24–72 h after replating the stable cells. Cells were clamped at –60 mV and drugs were delivered via local rapid perfusion for 500 ms, as described in Materials and methods section.