Table 2. Dose-Dependent Effects of Diazepam in the Social Fear Conditioning Paradigm and the Elevated Plus Maze (EPM).

Conditioning	Treatment	% NSI	% SI	% Sedative index	CA entries	Time OA
Unconditioned mice	Vehicle	23.6±1.7	69.6±2.5	0±0	100±5.6	100±11.0
	0.5 Dia	20.3±3.0	68.8±2.4	NA	88.5±10.9	97.8±6.0
	0.75 Dia	7.6±1.8*	40.1±3.2*	28.4±4.8*	99.5±7.7	120.2±9.0
	1.0 Dia	12.2±5.3*	31.8±12.2*	34.3±13.9*	75.1±11.5	151.1±66.0
	1.25 Dia	4.7±3.7*	7.9±2.3*	51.8±8.0*	115.6±13.8	238.7±34.3*
Conditioned mice	Vehicle	22.0±3.9	28.8±4.9	NA	—	—
	0.5 Dia	22.1±6.3	64.6±7.7*	NA	—	—
	0.75 Dia	18.3±4.6	27.0±12.2	NA	—	—
	1.0 Dia	8.4±2.3	19.8±8.8	NA	—	—
	1.25 Dia	13.9±7.1	10.1±5.8	NA	—	—

Abbreviations: CA, closed arms of the EPM; NA, not analyzed; NSI, non-social investigation; OA, open arms of the EPM; SFC, social fear conditioning; SI, social investigation.

Unconditioned and conditioned mice were injected intraperitoneally either with vehicle (5 ml/kg saline) or with diazepam (Dia; 0.5, 0.75, 1.0, or 1.25 mg/kg) 30 min before extinction in the SFC paradigm or EPM testing. Data represent mean values ±SEM. The sedative index is defined as the percentage of time lacking muscle tone and movement.

^*p<0.05.