Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1990 Dec 25;18(24):7229–7234. doi: 10.1093/nar/18.24.7229

Identification and purification of a Bombyx mori homologue of FTZ-F1.

H Ueda 1, S Hirose 1
PMCID: PMC332857  PMID: 2124348

Abstract

Extracts from embryos and from posterior and middle silk glands of the silkworm, Bombyx mori contain a sequence specific DNA binding factor termed BmFTZ-F1. The factor binds to the recognition site of FTZ-F1, a positive regulator of the fushi tarazu gene in Drosophila melanogaster. BmFTZ-F1 and FTZ-F1 share the same methylation interference patterns, the same chromatographic behaviors and similar protease digestion profiles. Anti-FTZ-F1 cross reacts with BmFTZ-F1. These results indicate that BmFTZ-F1 is a B. mori homologue of FTZ-F1. The mobility of the factor-DNA complex formed in the silk gland extract changes depending on the developmental stages. Purification of BmFTZF1 to an almost homogeneous state reveals that the factor is a 73 kd protein.

Full text

PDF
7229

Images in this article

7230Image
on p.7230
7231Image
on p.7231
7231Image
on p.7231
7232Image
on p.7232
7233Image
on p.7233

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akam M. The molecular basis for metameric pattern in the Drosophila embryo. Development. 1987 Sep;101(1):1–22. [PubMed] [Google Scholar]
  2. Blochlinger K., Bodmer R., Jack J., Jan L. Y., Jan Y. N. Primary structure and expression of a product from cut, a locus involved in specifying sensory organ identity in Drosophila. Nature. 1988 Jun 16;333(6174):629–635. doi: 10.1038/333629a0. [DOI] [PubMed] [Google Scholar]
  3. Carroll S. B., Scott M. P. Localization of the fushi tarazu protein during Drosophila embryogenesis. Cell. 1985 Nov;43(1):47–57. doi: 10.1016/0092-8674(85)90011-x. [DOI] [PubMed] [Google Scholar]
  4. Costa M., Weir M., Coulson A., Sulston J., Kenyon C. Posterior pattern formation in C. elegans involves position-specific expression of a gene containing a homeobox. Cell. 1988 Dec 2;55(5):747–756. doi: 10.1016/0092-8674(88)90131-6. [DOI] [PubMed] [Google Scholar]
  5. Doe C. Q., Hiromi Y., Gehring W. J., Goodman C. S. Expression and function of the segmentation gene fushi tarazu during Drosophila neurogenesis. Science. 1988 Jan 8;239(4836):170–175. doi: 10.1126/science.2892267. [DOI] [PubMed] [Google Scholar]
  6. Finney M., Ruvkun G., Horvitz H. R. The C. elegans cell lineage and differentiation gene unc-86 encodes a protein with a homeodomain and extended similarity to transcription factors. Cell. 1988 Dec 2;55(5):757–769. doi: 10.1016/0092-8674(88)90132-8. [DOI] [PubMed] [Google Scholar]
  7. Ingham P. W. The molecular genetics of embryonic pattern formation in Drosophila. Nature. 1988 Sep 1;335(6185):25–34. doi: 10.1038/335025a0. [DOI] [PubMed] [Google Scholar]
  8. Kondo K., Aoshima Y., Hagiwara T., Ueda H., Mizuno S. Tissue-specific and periodic changes in the nuclease sensitivity of the fibroin gene chromatin in the silkworm Bombyx mori. J Biol Chem. 1987 Apr 15;262(11):5271–5279. [PubMed] [Google Scholar]
  9. Krause H. M., Klemenz R., Gehring W. J. Expression, modification, and localization of the fushi tarazu protein in Drosophila embryos. Genes Dev. 1988 Aug;2(8):1021–1036. doi: 10.1101/gad.2.8.1021. [DOI] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Lassar A. B., Buskin J. N., Lockshon D., Davis R. L., Apone S., Hauschka S. D., Weintraub H. MyoD is a sequence-specific DNA binding protein requiring a region of myc homology to bind to the muscle creatine kinase enhancer. Cell. 1989 Sep 8;58(5):823–831. doi: 10.1016/0092-8674(89)90935-5. [DOI] [PubMed] [Google Scholar]
  12. Maekawa H., Suzuki Y. Repeated turn-off and turn-on of fibroin gene transcription during silk gland development of Bombyx mori. Dev Biol. 1980 Aug;78(2):394–406. doi: 10.1016/0012-1606(80)90343-7. [DOI] [PubMed] [Google Scholar]
  13. Matsuno K., Suzuki T., Takiya S., Suzuki Y. Complex formation with the fibroin gene enhancer through a protein-protein interaction analyzed by a modified DNA-binding assay. J Biol Chem. 1989 Mar 15;264(8):4599–4604. [PubMed] [Google Scholar]
  14. Obara T., Suzuki Y. Temporal and spatial control of silk gene transcription analyzed by nuclear run-on assays. Dev Biol. 1988 Jun;127(2):384–391. doi: 10.1016/0012-1606(88)90325-9. [DOI] [PubMed] [Google Scholar]
  15. Saint R., Kalionis B., Lockett T. J., Elizur A. Pattern formation in the developing eye of Drosophila melanogaster is regulated by the homoeo-box gene, rough. Nature. 1988 Jul 14;334(6178):151–154. doi: 10.1038/334151a0. [DOI] [PubMed] [Google Scholar]
  16. Scott M. P., Carroll S. B. The segmentation and homeotic gene network in early Drosophila development. Cell. 1987 Dec 4;51(5):689–698. doi: 10.1016/0092-8674(87)90092-4. [DOI] [PubMed] [Google Scholar]
  17. Suzuki T., Suzuki Y. Interaction of composite protein complex with the fibroin enhancer sequence. J Biol Chem. 1988 Apr 25;263(12):5979–5986. [PubMed] [Google Scholar]
  18. Suzuki Y., Giza P. E. Accentuated expression of silk fibroin genes in vivo and in vitro. J Mol Biol. 1976 Nov 5;107(3):183–206. doi: 10.1016/s0022-2836(76)80001-0. [DOI] [PubMed] [Google Scholar]
  19. Suzuki Y., Tsuda M., Takiya S., Hirose S., Suzuki E., Kameda M., Ninaki O. Tissue-specific transcription enhancement of the fibroin gene characterized by cell-free systems. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9522–9526. doi: 10.1073/pnas.83.24.9522. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Takiya S., Suzuki Y. Factors involved in preferential transcription of the fibroin gene. Eur J Biochem. 1989 Jan 15;179(1):1–9. doi: 10.1111/j.1432-1033.1989.tb14513.x. [DOI] [PubMed] [Google Scholar]
  21. Tsuda M., Suzuki Y. Faithful transcription initiation of fibroin gene in a homologous cell-free system reveals an enhancing effect of 5' flanking sequence far upstream. Cell. 1981 Nov;27(1 Pt 2):175–182. doi: 10.1016/0092-8674(81)90371-8. [DOI] [PubMed] [Google Scholar]
  22. Wakimoto B. T., Kaufman T. C. Analysis of larval segmentation in lethal genotypes associated with the antennapedia gene complex in Drosophila melanogaster. Dev Biol. 1981 Jan 15;81(1):51–64. doi: 10.1016/0012-1606(81)90347-x. [DOI] [PubMed] [Google Scholar]
  23. Way J. C., Chalfie M. mec-3, a homeobox-containing gene that specifies differentiation of the touch receptor neurons in C. elegans. Cell. 1988 Jul 1;54(1):5–16. doi: 10.1016/0092-8674(88)90174-2. [DOI] [PubMed] [Google Scholar]
  24. Wu C., Wilson S., Walker B., Dawid I., Paisley T., Zimarino V., Ueda H. Purification and properties of Drosophila heat shock activator protein. Science. 1987 Nov 27;238(4831):1247–1253. doi: 10.1126/science.3685975. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES