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. 1990 Dec 25;18(24):7279–7286. doi: 10.1093/nar/18.24.7279

Synthesis and characterization of a substrate for T4 endonuclease V containing a phosphorodithioate linkage at the thymine dimer site.

T Murata 1, S Iwai 1, E Ohtsuka 1
PMCID: PMC332863  PMID: 2259623

Abstract

A dodecadeoxyribonucleotide containing a cis-syn thymine dimer with a phosphorodithioate linkage was synthesized on a solid support using a dinucleotide coupling unit prepared by UV-irradiation of dithymidine monophosphorodithioate followed by S- and 5'-O-protection and 3'-phosphitylation. A photodimer-containing dodecamer without phosphate modification was also synthesized. The dodecamers were hybridized to the complementary sequence, and the duplexes used as substrates for T4 endonuclease V. This enzyme cleaved the phosphate-modified substrate more slowly than the unmodified duplex with the same dissociation constant.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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