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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Shock. 2012 May;37(5):10.1097/SHK.0b013e318249cfa2. doi: 10.1097/SHK.0b013e318249cfa2

Figure 2. IRF-1 KO mice have decreased apoptosis in splenocytes following LPS exposure.

Figure 2

Permanent blocks of splenic tissue obtained from WT and IRF-1 KO mice 16 h following PBS or LPS (20 mg/kg) injection were sectioned and (A) TUNEL staining or (B) cleaved caspase-3 immunohistochemistry staining was performed (magnitude X 200). Positive staining was presented as percentage of stained area over total area (% area stained). (C) Splenic tissue obtained from WT and IRF-1 KO mice 16 h following PBS or LPS (20 mg/kg) injection was imaged by transmission electron microscope (magnitude X 5000). Arrow points to apoptotic bodies. Percentage of apoptotic cells among total cells was used for apoptosis quantification. (D) WT and IRF-1 KO mice were injected with PBS or LPS (20 mg/kg) for 16 h and splenocytes were isolated. Caspase-3 cleavage was analyzed by Western blot. *, P<0.05; results are representative of 3 separate independent experiments.