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. 1972 Jan;51(1):22–30. doi: 10.1172/JCI106792

Renin Activation in the Venous Plasma from the Involved Kidney in the Patient with Renal Hypertension

Mohinder P Sambhi 1, Charles E Wiedeman 1
PMCID: PMC332924  PMID: 4331798

Abstract

Measurements of plasma renin activity (PRA) in renal vein blood from the ischemic kidney are reported to be generally higher than from the contralateral kidney. Importance of factors other than renin content of renal venous plasma has not been investigated. Initial rate measurements of angiotensin generation with added excess of homologous renin (Plasma Renin Substrate Activity [PRSA]-20 min) were made in bilateral renal venous plasma from 31 patients suspected of suffering from unilateral renal hypertension. The mean values from the involved vs. the contralateral kidney were 551 vs. 331 ng respectively of angiotensin II equivalents generated per milliliter of plasma per 20 min of incubation. The measurement of maximal angiotensin generation under the same conditions with incubation prolonged to 3 hr (PRSA-180 min), however, were bilaterally equal in renal venous plasma from selected patients with renal hypertension who showed distinct differences in PRSA-20 min and PRA measurements. Prior extraction of plasma lipids did not significantly change the bilateral renal venous PRSA-20 min determinations. Stimulation of endogenous renin release in normal dogs did not change the PRSA determinations. The data suggest strongly the presence of a “renin activating” mechanism in the renal venous plasma from the involved kidney of patients with renal hypertension.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amsterdam E. A., Couch N. P., Christlieb A. R., Harrison J. H., Crane C., Dobrzinsky S. J., Hickler R. B. Renal vein renin activity in the prognosis of surgery for renovascular hypertension. Am J Med. 1969 Dec;47(6):860–868. doi: 10.1016/0002-9343(69)90201-0. [DOI] [PubMed] [Google Scholar]
  2. BOUCHER R., VEYRAT R., DE CHAMPLAIN J., GENEST J. NEW PROCEDURES FOR MEASUREMENT OF HUMAN PLASMA ANGIOTENSIN AND RENIN ACTIVITY LEVELS. Can Med Assoc J. 1964 Jan 25;90:194–201. [PMC free article] [PubMed] [Google Scholar]
  3. Bath N. M., Gunnells J. C., Jr, Robinson R. R. Plasma renin activity in renovascular hypertension. Am J Med. 1968 Sep;45(3):381–390. doi: 10.1016/0002-9343(68)90072-7. [DOI] [PubMed] [Google Scholar]
  4. Bourgoignie J., Kurz S., Catanzaro F. J., Serirat P., Perry H. M., Jr Renal venous renin in hypertension. Am J Med. 1970 Mar;48(3):332–342. doi: 10.1016/0002-9343(70)90064-1. [DOI] [PubMed] [Google Scholar]
  5. Fitz A. Renal venous renin determinations in the diagnosis of surgically correctable hypertension. Circulation. 1967 Dec;36(6):942–950. doi: 10.1161/01.cir.36.6.942. [DOI] [PubMed] [Google Scholar]
  6. Gocke D. J., Gerten J., Sherwood L. M., Laragh J. H. Physiological and pathological variations of plasma angiotensin II in man. Correlation with renin activity and sodium balance. Circ Res. 1969 May;24(5 Suppl):131–148. [PubMed] [Google Scholar]
  7. Gould A. B., Skeggs L. T., Kahn J. R. Measurement of renin and substrate concentrations in human serum. Lab Invest. 1966 Nov;15(11):1802–1813. [PubMed] [Google Scholar]
  8. Grollman A., Ebihara A. Comparison of the pressor activity and the renin content of the renal venous effluent in patients suspected of suffering from surgically remediable hypertension. Tex Rep Biol Med. 1968 Fall;26(3):313–319. [PubMed] [Google Scholar]
  9. Haas E., Goldblatt H., Gipson E. C., Lewis L. Extraction, purification, and assay of human renin free of angiotensinase. Circ Res. 1966 Oct;19(4):739–749. doi: 10.1161/01.res.19.4.739. [DOI] [PubMed] [Google Scholar]
  10. Meyer P., Ecoiffier J., Alexandre J. M., Devaux C., Guize L., Menard J., Biron P., Milliez P. Prognostic value of plasma renin activity in renovascular hypertension. Circulation. 1967 Oct;36(4):570–576. doi: 10.1161/01.cir.36.4.570. [DOI] [PubMed] [Google Scholar]
  11. Michelakis A. M., Foster J. H., Liddle G. W., Rhamy R. K., Kuchel O., Gordon R. D. Measurement of renin in both renal veins. Its use in diagnosis of renovascular hypertension. Arch Intern Med. 1967 Oct;120(4):444–448. [PubMed] [Google Scholar]
  12. Osmond D. H., Smeby R. R., Bumpus F. M. Quantitative studies of renin preinhibitor and total phospholipids in organs and in plasma and erythrocytes to control, nephrectomized, and very old rats. J Lab Clin Med. 1969 May;73(5):795–808. [PubMed] [Google Scholar]
  13. Ostrovsky D., Sen S., Smeby R. R., Bumpus F. M. Chemical assay of phospholipid renin preinhibitor in canine and human blood. Circ Res. 1967 Oct;21(4):497–505. doi: 10.1161/01.res.21.4.497. [DOI] [PubMed] [Google Scholar]
  14. Pickens P. T., Bumpus F. M., Lloyd A. M., Smeby R. R., Page I. H. Measurement of renin activity in human plasma. Circ Res. 1965 Nov;17(5):438–448. doi: 10.1161/01.res.17.5.438. [DOI] [PubMed] [Google Scholar]
  15. Sambhi M. P., Barrett J. D. Development of tachyphylaxis to aspartyl and not asparginyl angiotensin II in the rat. Circ Res. 1967 Sep;21(3):327–332. doi: 10.1161/01.res.21.3.327. [DOI] [PubMed] [Google Scholar]
  16. Sen S., Smeby R. R., Bumpus F. M. Isolation of a phospholipid renin inhibitor from kidney. Biochemistry. 1967 Jun;6(6):1572–1581. doi: 10.1021/bi00858a002. [DOI] [PubMed] [Google Scholar]
  17. Shapiro A. P., Perez-Stable E., Scheib E. T., Bron K., Moutsos S. E., Berg G., Misage J. R. Renal artery stenosis and hypertension. Observations on current status of therapy from a study of 115 patients. Am J Med. 1969 Aug;47(2):175–193. doi: 10.1016/0002-9343(69)90145-4. [DOI] [PubMed] [Google Scholar]
  18. Skinner S. L. Improved assay methods for renin "concentration" and "activity" in human plasma. Methods using selective denaturation of renin substrate. Circ Res. 1967 Apr;20(4):391–402. doi: 10.1161/01.res.20.4.391. [DOI] [PubMed] [Google Scholar]

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