Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1974 Mar;53(3):735–744. doi: 10.1172/JCI107612

Cell-Mediated Immune Response of Ragweed-Sensitive Patients to Ragweed Antigen E

IN VITRO LYMPHOCYTE TRANSFORMATION AND ELABORATION OF LYMPHOCYTE MEDIATORS

Ross E Rocklin 1, Hobert Pence 1, Hyman Kaplan 1, Richard Evans 1
PMCID: PMC333054  PMID: 4130213

Abstract

The in vivo and in vitro responses to ragweed antigen E were evaluated in 28 untreated atopic patients with ragweed hayfever. The methods employed included direct skin testing, measurement of total serum IgE, measurement of specific IgE anti-ragweed antibodies, leukocyte histamine release, lymphocyte transformation, and release of lymphocyte mediators (migration inhibitory factor and mitogenic factor). The patients could be divided into sensitive and insensitive groups on the basis of their in vitro reactivity to antigen E. 20 patients in the sensitive group had statistically higher levels of total serum IgE, higher levels of specific IgE anti-ragweed antibodies, and greater leukocyte sensitivity as measured by antigen-induced histamine release than did eight patients in the insensitive group. Lymphocytes from sensitive patients produced greater amounts of migration inhibitory factor and mitogenic factor when challenged by antigen E than did lymphocytes from insensitive patients. A possible role for the lymphocyte in this allergic disease is discussed. The results of this study indicate that the immune response to ragweed antigen is complex and involves components of both immediate and delayed hypersensitivity.

Full text

PDF
735

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brostoff J., Roitt I. M. Cell-mediated (delayed) hypersensitivity in patients with summer hay-fever. Lancet. 1969 Dec 13;2(7633):1269–1272. doi: 10.1016/s0140-6736(69)90807-1. [DOI] [PubMed] [Google Scholar]
  2. Churchill W. H., Rocklin R. R., Moloney W. C., David J. R. In vitro evidence of normal lymphocyte function in some patients with Hodgkin's disease and negative delayed cutaneous hypersensitivity. Natl Cancer Inst Monogr. 1973 May;36:99–106. [PubMed] [Google Scholar]
  3. DAVID J. R., AL-ASKARI S., LAWRENCE H. S., THOMAS L. DELAYED HYPERSENSITIVITY IN VITRO. I. THE SPECIFICITY OF INHIBITION OF CELL MIGRATION BY ANTIGENS. J Immunol. 1964 Aug;93:264–273. [PubMed] [Google Scholar]
  4. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Girard J. P., Rose N. R., Kunz M. L., Kobayashi S., Arbesman C. E. In vitro lymphocyte transformation in atopic patients: induced by antigens. J Allergy. 1967 Feb;39(2):65–81. doi: 10.1016/0021-8707(67)90113-x. [DOI] [PubMed] [Google Scholar]
  6. Gleich G. J., Averbeck A. K., Swedlund H. A. Measurement of IgE in normal and allergic serum by radioimmunoassay. J Lab Clin Med. 1971 Apr;77(4):690–698. [PubMed] [Google Scholar]
  7. Go S., Michaels D. L., Ellis E. F. Delayed hypersensitivity to pollen. Lancet. 1970 May 23;1(7656):1115–1116. doi: 10.1016/s0140-6736(70)92784-4. [DOI] [PubMed] [Google Scholar]
  8. Green G. R., Zweiman B., Beerman H., Hildreth E. A. Delayed skin reactions to inhalant antigens. J Allergy. 1967 Oct;40(4):224–236. doi: 10.1016/0021-8707(67)90085-8. [DOI] [PubMed] [Google Scholar]
  9. Henderson L. L., Swedlund H. A., Van Dellen R. G., Marcoux J. P., Carryer H. M., Peters G. A., Gleich G. J. Evaluation of IgE tests in an allergy practice. J Allergy Clin Immunol. 1971 Dec;48(6):361–365. doi: 10.1016/0091-6749(71)90082-0. [DOI] [PubMed] [Google Scholar]
  10. Kishimoto T., Ishizaka K. Regulation of antibody response in vitro. V. Effect of carrier-specific Helper cells on generation of hapten-specific memory cells of different immunoglobulin classes. J Immunol. 1973 Jul;111(1):1–9. [PubMed] [Google Scholar]
  11. LICHTENSTEIN L. M., OSLER A. G. STUDIES ON THE MECHANISMS OF HYPERSENSITIVITY PHENOMENA. IX. HISTAMINE RELEASE FROM HUMAN LEUKOCYTES BY RAGWEED POLLEN ANTIGEN. J Exp Med. 1964 Oct 1;120:507–530. doi: 10.1084/jem.120.4.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Levy D. A., Lichtenstein L. M., Goldstein E. O., Ishizaka K. Immunologic and cellular changes accompanying the therapy of pollen allergy. J Clin Invest. 1971 Feb;50(2):360–369. doi: 10.1172/JCI106503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lichtenstein L. M., Ishizaka K., Norman P. S., Sobotka A. K., Hill B. M. IgE antibody measurements in ragweed hay fever. Relationship to clinical severity and the results of immunotherapy. J Clin Invest. 1973 Feb;52(2):472–482. doi: 10.1172/JCI107204. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lichtenstein L. M., Norman P. S., Winkenwerder W. L., Osler A. G. In vitro studies of human ragweed allergy: changes in cellular and humoral activity associated with specific desensitization. J Clin Invest. 1966 Jul;45(7):1126–1136. doi: 10.1172/JCI105419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Maini R. N., Dumonde D. C., Faux J. A., Hargreave F. E., Pepys J. The production of lymphocyte mitogenic factor and migration-inhibition factor by antigen-stimulated lymphocytes of subjects with grass pollen allergy. Clin Exp Immunol. 1971 Oct;9(4):449–465. [PMC free article] [PubMed] [Google Scholar]
  16. Massie F. S., Wutanasupta R. Detection of delayed hypersensitivity in ragweed pollinosis by an in vitro method. Ann Allergy. 1972 Dec;30(12):676–684. [PubMed] [Google Scholar]
  17. May C. D., Lyman M., Alberto R., Cheng J. Procedures for immunochemical study of histamine release from leukocytes with small volume of blood. J Allergy. 1970 Jul;46(1):12–20. doi: 10.1016/0021-8707(70)90056-0. [DOI] [PubMed] [Google Scholar]
  18. Okumura K., Tada T. Regulation of homocytotropic antibody formation in the rat. 3. Effect of thymectomy and splenectomy. J Immunol. 1971 Apr;106(4):1019–1025. [PubMed] [Google Scholar]
  19. Richter M., Naspitz C. K. The in vitro blastogenic response of lymphocytes of ragweed-sensitive individuals. J Allergy. 1968 Mar;41(3):140–151. doi: 10.1016/0021-8707(68)90054-3. [DOI] [PubMed] [Google Scholar]
  20. Rocklin R. E., Meyers O. L., David J. R. An in vitro assay for cellular hypersensitivity in man. J Immunol. 1970 Jan;104(1):95–102. [PubMed] [Google Scholar]
  21. SHORE P. A., BURKHALTER A., COHN V. H., Jr A method for the fluorometric assay of histamine in tissues. J Pharmacol Exp Ther. 1959 Nov;127:182–186. [PubMed] [Google Scholar]
  22. SLAVIN R. G., TENNENBAUM J. I., BECKER R. J., FEINBERG A. R., FEINBERG S. M. CELL TRANSFER OF DELAYED HYPERSENSITIVITY TO RAGWEED FROM ATOPIC SUBJECTS TREATED WITH EMULSIFIED RAGWEED EXTRACTS. J Allergy. 1963 Jul-Aug;34:368–373. doi: 10.1016/0021-8707(63)90055-8. [DOI] [PubMed] [Google Scholar]
  23. Spitz E., Gelfand E. W., Sheffer A. L., Austen K. F. Serum IgE in clinical immunology and allergy. J Allergy Clin Immunol. 1972 Jun;49(6):337–347. doi: 10.1016/0091-6749(72)90132-7. [DOI] [PubMed] [Google Scholar]
  24. Wide L., Bennich H., Johansson S. G. Diagnosis of allergy by an in-vitro test for allergen antibodies. Lancet. 1967 Nov 25;2(7526):1105–1107. doi: 10.1016/s0140-6736(67)90615-0. [DOI] [PubMed] [Google Scholar]
  25. Yunginger J. W., Gleich G. J. Comparison of the protein-binding capacities of cyanogen bromide-activated polysaccharides. J Allergy Clin Immunol. 1972 Aug;50(2):109–116. doi: 10.1016/0091-6749(72)90006-1. [DOI] [PubMed] [Google Scholar]
  26. Zeitz S. J., Van Arsdel P. P., Jr, McClure D. K. Specific response of human lymphocytes to pollen antigen in tissue culture. J Allergy. 1966 Dec;38(6):321–329. doi: 10.1016/0021-8707(66)90030-x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES