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. 1974 Apr;53(4):1202–1205. doi: 10.1172/JCI107660

Studies on the Mode of Action of Cholera Toxin

EFFECTS ON SOLUBILIZED ADENYLATE CYCLASE

Barbara Beckman 1,2,3, Jorge Flores 1,2,3, Patricia A Witkum 1,2,3, Geoffrey W G Sharp 1,2,3
PMCID: PMC333108  PMID: 4815085

Abstract

To gain further insight into the mechanism of action of cholera toxin, solubilized preparations of adenylate cyclase from control and toxin-treated rat livers were studied. Adenylate cyclase activity was measured in both particulate and solubilized form in rat liver under control conditions and after intravenous injection of cholera toxin. Cholera toxin caused a 3.3-fold activation of adenylate cyclase in the particulate preparation and a 5.8-fold increase in the solubilized preparation. Thus, the ability of cholera toxin to stimulate adenylate cyclase is present even when the enzyme membrane environment is disrupted. Furthermore, the solubilized enzyme, after treatment with cholera toxin, retained its ability to respond to catecholamines, but not to glucagon. In contrast, the control enzyme lost its responsiveness to catecholamines and glucagon after solubilization.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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