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. 1976 Jul;58(1):109–122. doi: 10.1172/JCI108439

Suppression of B-cell differentiation by leukocytes from hypogammaglobulinemic patients.

F P Siegal, M Siegal, R A Good
PMCID: PMC333161  PMID: 1084353

Abstract

Leukocytes from patients with several forms of immunodeficiency characterized by apparently differing defects in B-lymphocyte maturation produced few or no plasmacytoid cells in vitro, and were capable of suppressing the generation of plasma cells in co-culture with cells of normal persons, in the presence of pokeweed mitogen. Such inhibition was commonly observed in cultures which included cells from patients with primary immunoglobulin deficiency, but was not seen to a significant degree in identical co-cultures of cells from normals. The suppression observed was not dependent upon mixed leukocyte culture reactivity. Both sheep erythrocyte-rosetting lymphocytes and adherent cells appeared to participate in these effects in some patients. In one patient with common variable immunodeficiency, but not in several others, removal of suppressing cells permitted the patient's remaining cells to differentiate into plasma cells in vitro. Because of the diverse syndromes in which suppression was observed, it is likely that, in at least some hypogammaglobulinemic patients, the suppression is secondary to the disease process rather than being the primary pathogenic mechanism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bentwich Z., Douglas S. D., Siegal F. P., Kunkel H. G. Human lymphocyte-sheep erythrocyte rosette formation: some characteristics of the interaction. Clin Immunol Immunopathol. 1973 Jul;1(4):511–522. doi: 10.1016/0090-1229(73)90007-x. [DOI] [PubMed] [Google Scholar]
  2. Blaese R. M., Weiden P. L., Koski I., Dooley N. Infectious agammaglobulinemia: transmission of immunodeficiency with grafts of agammaglobulinemic cells. J Exp Med. 1974 Oct 1;140(4):1097–1101. doi: 10.1084/jem.140.4.1097. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Broom B. C., De la Concha E. G., Webster A. D., Janossy G. J., Asherson G. L. Intracellular immunoglobulin production in vitro by lymphocytes from patients with hypogammaglobulinaemia and their effect on normal lymphocytes. Clin Exp Immunol. 1976 Jan;23(1):73–77. [PMC free article] [PubMed] [Google Scholar]
  4. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  5. Choi Y. S., Biggar W. D., Good R. A. Biosynthesis and secretion of immunoglobulins by peripheral-blood lymphocytes in severe hypogammaglobulinaemia. Lancet. 1972 May 27;1(7761):1149–1152. doi: 10.1016/s0140-6736(72)91374-8. [DOI] [PubMed] [Google Scholar]
  6. Cooper M. D., Faulk W. P., Fudenberg H. H., Good R. A., Hitzig W., Kunkel H., Rosen F. S., Seligmann M., Soothill J., Wedgwood R. J. Classification of primary immunodeficiencies. N Engl J Med. 1973 May 3;288(18):966–967. doi: 10.1056/NEJM197305032881814. [DOI] [PubMed] [Google Scholar]
  7. Cooper M. D., Keightley R. G., Wu L. Y., Lawton A. R., 3rd Developmental defects of T and B cell lines in humans. Transplant Rev. 1973;16:51–84. doi: 10.1111/j.1600-065x.1973.tb00117.x. [DOI] [PubMed] [Google Scholar]
  8. Crabbé P. A., Heremans J. F. The distribution of immunoglobulin-containing cells along the human gastrointestinal tract. Gastroenterology. 1966 Sep;51(3):305–316. [PubMed] [Google Scholar]
  9. Douglas S. D. Human lymphocyte growth in vitro: morphologic, biochemical, and immunologic significance. Int Rev Exp Pathol. 1971;10:41–114. [PubMed] [Google Scholar]
  10. Folch H., Waksman B. H. Regulation of lymphocyte responses in vitro. V. Suppressor activity of adherent and nonadherent rat lymphoid cells. Cell Immunol. 1973 Oct;9(1):12–24. doi: 10.1016/0008-8749(73)90163-9. [DOI] [PubMed] [Google Scholar]
  11. Froland S. S., Natvig J. B. Identification of three different human lymphocyte populations by surface markers. Transplant Rev. 1973;16:114–162. doi: 10.1111/j.1600-065x.1973.tb00119.x. [DOI] [PubMed] [Google Scholar]
  12. Fudenberg H., Good R. A., Goodman H. C., Hitzig W., Kunkel H. G., Roitt I. M., Rosen F. S., Rowe D. S., Seligmann M., Soothill J. R. Primary immunodeficiencies. Report of a World Health Organization Committee. Pediatrics. 1971 May;47(5):927–946. [PubMed] [Google Scholar]
  13. GOOD R. A. Studies on agammaglobulinemia. II. Failure of plasma cell formation in the bone marrow and lymph nodes of patients with agammaglobulinemia. J Lab Clin Med. 1955 Aug;46(2):167–181. [PubMed] [Google Scholar]
  14. Geha R. S., Schneeberger E., Merler E., Rosen F. S. Heterogeneity of "acquired" or common variable agammaglobulinemia. N Engl J Med. 1974 Jul 4;291(1):1–6. doi: 10.1056/NEJM197407042910101. [DOI] [PubMed] [Google Scholar]
  15. Gershon R. K. T cell control of antibody production. Contemp Top Immunobiol. 1974;3:1–40. doi: 10.1007/978-1-4684-3045-5_1. [DOI] [PubMed] [Google Scholar]
  16. Siegal F. P., Wernet P., Dickler H. B., Fu S. M., Kunkel H. G. B lymphocytes lacking surface IG in patients with immune deficiency: initiation of IG synthesis in culture in cells of a patient with thymoma. Birth Defects Orig Artic Ser. 1975;11(1):40–44. [PubMed] [Google Scholar]
  17. Waldmann T. A., Durm M., Broder S., Blackman M., Blaese R. M., Strober W. Role of suppressor T cells in pathogenesis of common variable hypogammaglobulinaemia. Lancet. 1974 Sep 14;2(7881):609–613. doi: 10.1016/s0140-6736(74)91940-0. [DOI] [PubMed] [Google Scholar]
  18. Wu L. Y., Lawton A. R., Cooper M. D. Differentiation capacity of cultured B lymphocytes from immunodeficient patients. J Clin Invest. 1973 Dec;52(12):3180–3189. doi: 10.1172/JCI107518. [DOI] [PMC free article] [PubMed] [Google Scholar]

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