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. 1976 Aug;58(2):380–390. doi: 10.1172/JCI108482

The effects of sodium ricinoleate on small intestinal function and structure.

W S Cline, V Lorenzsonn, L Benz, P Bass, W A Olsen
PMCID: PMC333193  PMID: 956372

Abstract

The mechanism of hydroxy fatty acid-induced secretion was investigated in perfused hamster small intestine in vivo. Sodium ricinoleate at an 8-mM concentration resulted in not only secretion of water and sodium, but an increase in intestinal clearance of inulin and a 16,000 mol wt dextran as well. A concentration of ricinoleate (2 mM) which did not affect water transport, however, did not alter intestinal permeability. Ricinoleate-induced intestinal secretion was also accompanied by increased mucosal cell exfoliation as measured by the appearance of DNA in the perfusate and by apparent injury to epithelial cell membranes as judged by measurement of sucrase activity and phospholipid in cell-free aliquots of luminal fluid. Light and electron microscopic studies demonstrated substantial mucosal architectural changes with 8 mM ricinoleate with villus shortening and injury to epithelial cells at the villus tips. In contrast, cholera enterotoxin caused marked secretion of sodium and water, presumably by a cyclic AMP mechanism, but did not alter inulin clearance or enhance DNA or sucrase appearance in the lumen. These studies suggest that at least a component of ricinoleate-induced intestinal secretion is related to structural alterations of the mucosa.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ammon H. V., Phillips S. F. Inhibition of colonic water and electrolyte absorption by fatty acids in man. Gastroenterology. 1973 Nov;65(5):744–749. [PubMed] [Google Scholar]
  2. Ammon H. V., Phillips S. F. Inhibition of ileal water absorption by intraluminal fatty acids. Influence of chain length, hydroxylation, and conjugation of fatty acids. J Clin Invest. 1974 Jan;53(1):205–210. doi: 10.1172/JCI107539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ammon H. V., Thomas P. J., Phillips S. F. Effects of oleic and ricinoleic acids on net jejunal water and electrolyte movement. Perfusion studies in man. J Clin Invest. 1974 Feb;53(2):374–379. doi: 10.1172/JCI107569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  5. Bright-Asare P., Binder H. J. Stimulation of colonic secretion of water and electrolytes by hydroxy fatty acids. Gastroenterology. 1973 Jan;64(1):81–88. [PubMed] [Google Scholar]
  6. CROFT D. N., LUBRAN M. THE ESTIMATION OF DEOXYRIBONUCLEIC ACID IN THE PRESENCE OF SIALIC ACID: APPLICATION TO ANALYSIS OF HUMAN GASTRIC WASHINGS. Biochem J. 1965 Jun;95:612–620. doi: 10.1042/bj0950612. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Christensen J., Freeman B. W. Circular muscle electromyogram in the cat colon: local effect of sodium ricinoleate. Gastroenterology. 1972 Dec;63(6):1011–1015. [PubMed] [Google Scholar]
  8. Christensen J., Weisbrodt N. W., Hauser R. L. Electrical slow wave of the proximal colon of the cat in diarrhea. Gastroenterology. 1972 Jun;62(6):1167–1173. [PubMed] [Google Scholar]
  9. DOGGENWEILER C. F., FRENK S. STAINING PROPERTIES OF LANTHANUM ON CELL MEMBRANES. Proc Natl Acad Sci U S A. 1965 Feb;53:425–430. doi: 10.1073/pnas.53.2.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dahlqvist A. Assay of intestinal disaccharidases. Anal Biochem. 1968 Jan;22(1):99–107. doi: 10.1016/0003-2697(68)90263-7. [DOI] [PubMed] [Google Scholar]
  11. Field M: Intestinal secretion: effect of cyclic AMP and its role in cholera. N Engl J Med. 1971 May 20;284(20):1137–1144. doi: 10.1056/NEJM197105202842008. [DOI] [PubMed] [Google Scholar]
  12. Friend D. S., Gilula N. B. Variations in tight and gap junctions in mammalian tissues. J Cell Biol. 1972 Jun;53(3):758–776. doi: 10.1083/jcb.53.3.758. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gaginella T. S., Stewart J. J., Gullikson G. W., Olsen W. A., Bass P. Inhibition of small intestinal mucosal and smooth muscle cell function by ricinoleic acid and other surfactants. Life Sci. 1975 May 15;16(10):1595–1605. doi: 10.1016/0024-3205(75)90077-6. [DOI] [PubMed] [Google Scholar]
  14. Gaginella T. S., Stewart J. J., Olsen W. A., Bass P. Actions of ricinoleic acid and structurally related fatty acids on the gastrointestinal tract. II. Effects on water and electrolyte absorption in vitro. J Pharmacol Exp Ther. 1975 Nov;195(2):355–361. [PubMed] [Google Scholar]
  15. Helenius A., Simons K. Solubilization of membranes by detergents. Biochim Biophys Acta. 1975 Mar 25;415(1):29–79. doi: 10.1016/0304-4157(75)90016-7. [DOI] [PubMed] [Google Scholar]
  16. Hofmann A. F., Poley J. R. Role of bile acid malabsorption in pathogenesis of diarrhea and steatorrhea in patients with ileal resection. I. Response to cholestyramine or replacement of dietary long chain triglyceride by medium chain triglyceride. Gastroenterology. 1972 May;62(5):918–934. [PubMed] [Google Scholar]
  17. JAMES A. T., WEBB J. P., KELLOCK T. D. The occurrence of unusual fatty acids in faecal lipids from human beings with normal and abnormal fat absorption. Biochem J. 1961 Feb;78:333–339. doi: 10.1042/bj0780333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kellock T. D., Pearson J. R., Russell R. I., Walker J. G., Wiggins H. S. The incidence and clinical significance of faecal hydroxy fatty acids. Gut. 1969 Dec;10(12):1055–1055. [PubMed] [Google Scholar]
  19. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. LeVeen H. H., Borek B., Axelrod D. R., Johnson A. Cause and treatment of diarrhea following resection of the small intestine. Surg Gynecol Obstet. 1967 Apr;124(4):766–770. [PubMed] [Google Scholar]
  21. MASRI M. S., GOLDBLATT L. A., DE EDS F., KOHLER G. O. Relation of cathartic activity to structural modifications of ricinoleic acid of castor oil. J Pharm Sci. 1962 Oct;51:999–1002. doi: 10.1002/jps.2600511022. [DOI] [PubMed] [Google Scholar]
  22. Neaves W. B. Permeability of Sertoli cell tight junctions to lanthanum after ligation of ductus deferens and ductuli efferentes. J Cell Biol. 1973 Dec;59(3):559–572. doi: 10.1083/jcb.59.3.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Overton J. Localized lanthanum staining of the intestinal brush border. J Cell Biol. 1968 Aug;38(2):447–452. doi: 10.1083/jcb.38.2.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Powell D. W., Farris R. K., Carbonetto S. T. Theophylline, cyclic AMP, choleragen, and electrolyte transport by rabbit ileum. Am J Physiol. 1974 Dec;227(6):1428–1435. doi: 10.1152/ajplegacy.1974.227.6.1428. [DOI] [PubMed] [Google Scholar]
  25. Rohde J. E., Chen L. C. Permeability and selectivity of canine and human jejunum during cholera. Gut. 1972 Mar;13(3):191–196. doi: 10.1136/gut.13.3.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Stewart J. J., Gaginella T. S., Olsen W. A., Bass P. Inhibitory actions of laxatives on motility and water and electrolyte transport in the gastrointestinal tract. J Pharmacol Exp Ther. 1975 Feb;192(2):458–467. [PubMed] [Google Scholar]
  27. Teem M. V., Phillips S. F. Perfusion of the hamster jejunum with conjugated and unconjugated bile acids: inhibition of water absorption and effects on morphology. Gastroenterology. 1972 Feb;62(2):261–267. [PubMed] [Google Scholar]
  28. Yau W. M., Makhlouf G. M. Different effects of hormonal peptides and cyclic adenosine 3',5'-monophosphate on colonic transport in vitro. Gastroenterology. 1974 Oct;67(4):662–667. [PubMed] [Google Scholar]
  29. Zurier R. B., Patterson J. F., Levitanr Relationship between net water absorption and hexanoic acid absorption from the intact human jejunum. Am J Dig Dis. 1968 Feb;13(2):109–112. doi: 10.1007/BF02232952. [DOI] [PubMed] [Google Scholar]

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