Figure 1.

A) Alignment of the NH₂-terminus of band 3 from different mammalian erythrocytes. The experimentally established deoxyhemoglobin binding sites for human and murine band 3 are framed in boxes. The sequence alignment was performed using the CLUSTAL W program (http://workbench.sdsc.edu/). Asterisks (*) indicate complete sequence conservation; (:) - conservation of strong groups; (.) - conservation of weak groups. B) Description of the mutant cytoplasmic domains of band 3 used in this study. For simplicity, only the first 70 amino acids of the 398 residue mouse cdb3 are shown. Deletions are denoted as (del) and substitutions as (subst). The substituted residues are printed in bold letters.