Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1976 Oct;58(4):950–954. doi: 10.1172/JCI108548

Inhibition of lymphoproliferation by hyperlipoproteinemic plasma.

C C Waddell, O D Taunton, J J Twomey
PMCID: PMC333258  PMID: 184112

Abstract

Plasma for patients with primary type IV or V hyperlipoproteinemia inhibited [3H]thymidine incorporation by cultured mononuclear leukocytes. This previously unreported abnormality affected mononuclear leukocytes from patients with type IV or V hyperlipoproteinemia and from normal subjects. Patient cells incorporated [3H]thymidine normally when washed and incubated in medium containing normal plasma. Both spontaneous incorporation and stimulated incorporation in response to various mitogens and antigens were inhibited. The inhibitory effect was identified with the chylomicron and very low density lipoprotein fractions isolated from plasma and was concentration-dependent. Lectin used to stimulate cultured cells and [3H]thymidine used to measure responses were not bound to the lipoproteins in appreciable amounts. [3H]-Thymidine incorporation correlated well with morphologic evidence of lymphoproliferation. The mechanism of the inhibitory effect of type IV or V hyperlipoproteinemic plasma upon the response tested was not identified by may be related to interaction between lipoproteins and the cell membranes. We suggest that these lipoproteins may also interfere with the function of other cells.

Full text

PDF
950

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beaumont J. L., Carlson L. A., Cooper G. R., Fejfar Z., Fredrickson D. S., Strasser T. Classification of hyperlipidaemias and hyperlipoproteinaemias. Bull World Health Organ. 1970;43(6):891–915. [PMC free article] [PubMed] [Google Scholar]
  2. Benditt E. P., Benditt J. M. Evidence for a monoclonal origin of human atherosclerotic plaques. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1753–1756. doi: 10.1073/pnas.70.6.1753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bloch K. J., Maki D. G. Hyperviscosity syndromes associated with immunoglobulin abnormalities. Semin Hematol. 1973 Apr;10(2):113–124. [PubMed] [Google Scholar]
  4. Brown M. S., Goldstein J. L. Familial hypercholesterolemia: defective binding of lipoproteins to cultured fibroblasts associated with impaired regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity. Proc Natl Acad Sci U S A. 1974 Mar;71(3):788–792. doi: 10.1073/pnas.71.3.788. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brown W. V., Levy R. I., Fredrickson D. S. Studies of the proteins in human plasma very low density lipoproteins. J Biol Chem. 1969 Oct 25;244(20):5687–5694. [PubMed] [Google Scholar]
  6. Burch G. E. Editorial: Viruses and arteriosclerosis. Am Heart J. 1974 Apr;87(4):407–412. doi: 10.1016/0002-8703(74)90163-x. [DOI] [PubMed] [Google Scholar]
  7. Canales L., Middlemas R. O., 3rd, Louro J. M., South M. A. Immunological observations in chronic mucocutaneous candidiasis. Lancet. 1969 Sep 13;2(7620):567–571. doi: 10.1016/s0140-6736(69)90264-5. [DOI] [PubMed] [Google Scholar]
  8. Carlson L. A., Böttiger L. E. Ischaemic heart-disease in relation to fasting values of plasma triglycerides and cholesterol. Stockholm prospective study. Lancet. 1972 Apr 22;1(7756):865–868. doi: 10.1016/s0140-6736(72)90738-6. [DOI] [PubMed] [Google Scholar]
  9. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  10. Gaines J. D., Gilmer M. A., Remington J. S. Deficiency of lymphocyte antigen recognition in Hodgkin's disease. Natl Cancer Inst Monogr. 1973 May;36:117–121. [PubMed] [Google Scholar]
  11. HIRSCHHORN K., SCHREIBMAN R. R., BACH F. H., SILTZBACH L. E. IN-VITRO STUDIES OF LYMPHOCYTES FROM PATIENTS WITH SARCOIDOSIS AND LYMPHOPROLIFERATIVE DISEASES. Lancet. 1964 Oct 17;2(7364):842–843. doi: 10.1016/s0140-6736(64)90691-9. [DOI] [PubMed] [Google Scholar]
  12. Han S. H., Weiser R. S., Lin Y. C. Transformation of leprous lymphocytes by leprolin, tuberculin and phytohemagglutinin. Int J Lepr Other Mycobact Dis. 1971 Oct-Dec;39(4):789–795. [PubMed] [Google Scholar]
  13. Kerby G. R. Correlation of tuberculin skin reaction with in vitro lymphocyte transformation. Am Rev Respir Dis. 1968 May;97(5):904–908. doi: 10.1164/arrd.1968.97.5.904. [DOI] [PubMed] [Google Scholar]
  14. Rocklin R. E., MacDermott R. P., Chess L., Schlossman S. F., David J. R. Studies on mediator production by highly purified human T and B lymphocytes. J Exp Med. 1974 Nov 1;140(5):1303–1316. doi: 10.1084/jem.140.5.1303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Salel A. F., Riggs K., Mason D. T., Amsterdam E. A., Zelis R. The importance of type IV hyperlipoproteinemia as a predisposing factor in coronary artery disease. Am J Med. 1974 Dec;57(6):897–903. doi: 10.1016/0002-9343(74)90167-3. [DOI] [PubMed] [Google Scholar]
  16. Schellekens P. T., Eijsvoogel V. P. Lymphocyte transformation in vitro. I. Tissue culture conditions and quantitative measurements. Clin Exp Immunol. 1968 Jul;3(6):571–584. [PMC free article] [PubMed] [Google Scholar]
  17. Silk M. Effect of plasma from patients with carcinoma on in vitro lymphocyte transformation. Cancer. 1967 Dec;20(12):2088–2089. doi: 10.1002/1097-0142(196712)20:12<2088::aid-cncr2820201205>3.0.co;2-1. [DOI] [PubMed] [Google Scholar]
  18. Twomey J. J., Laughter A. H., Farrow S., Douglass C. C. Hodgkin's disease. An immunodepleting and immunosuppressive disorder. J Clin Invest. 1975 Aug;56(2):467–475. doi: 10.1172/JCI108113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Twomey J. J., Sharkey O., Jr, Brown J. A., Laughter A. H., Jordan P. H., Jr Cellular requirements for the mitotic response in allogeneic mixed leukocyte cultures. J Immunol. 1970 Apr;104(4):845–853. [PubMed] [Google Scholar]
  20. Twomey J. J., Waddell C. C., Krantz S., O'reilly R., L'esperance P., Good R. A. Chronic mucocutaneous candidiasis with macrophage dysfunction, a plasma inhibitor, and co-existent aplastic anemia. J Lab Clin Med. 1975 Jun;85(6):968–977. [PubMed] [Google Scholar]
  21. Wernet P., Kunkel H. G. Antibodies to a specific surface antigen of T cells in human sera inhibiting mixed leukocyte culture reactions. J Exp Med. 1973 Oct 1;138(4):1021–1026. doi: 10.1084/jem.138.4.1021. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES