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Introduction
The role of iNOS in MOF has been controversial, studies having presented contradicting results, however, the use of more specific inhibitors of iNOS may provide benefit.
Aim
To examine the effects of iNOS inhibition in our model of TAA induced MOF.
Method
MOF was induced by two intraperitoneal (IP) injections of TAA (500 mg/kg) eight hours apart. Three groups were studied, Group 1 receiving TAA only. Group 2 and 3 followed thr protocol for Group 1, however, Group 2 was pre-treated with the NO precursor L-Arginine (300 mg/kg IP) once daily and Group 3 was pre-treated with NO synthase inhibitor aminoguanidine (100 mg/kg SC) for three days.
Results
See Table. n=10 Mean ± SD.
Histology
The histological sections show markedly less organ damage in the aminoguanidine group (Group 3).
Conclusion
Inhibition of iNOS using aminoguanidine significantly improves the incidence of MOF and mortality in the TAA model of MOF.
Table.
| Groups | AST (iu/l) | Creatinine (umol/l) | Lactate mmol/l | Survival % | |||
|---|---|---|---|---|---|---|---|
| Time (h) | 24 | 48 | 24 | 48 | 24 | 48 | 96 |
| Control | 34± 10 | 41± 12 | 43± 13 | 51± 10 | 1.5± 0.3 | 1.4± 0.2 | 100 |
| TAA | 1653± 164 | 4045± 247 | 167± 22 | 231± 37 | 3.9± 0.9 | 6.2± 0.7 | 25 |
| TAA + L-ARG | 2177± 231 | 4475± 240 | 187± 14 | 271± 27 | 4.2± 0.5 | 7.3± 0.7 | 10 |
| TAA + AMG | 346± 50 | 1090± 188 | 87± 21 | 112± 23 | 2.1± 0.2 | 3.4± 0.3 | 90 |