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. 1977 Jan;59(1):117–124. doi: 10.1172/JCI108608

Inhibition of adherence of Neisseria gonorrhoeae by human genital secretions.

E C Tramont
PMCID: PMC333338  PMID: 401507

Abstract

Local genital antibodies to the infecting strains of Neisseria gonorrhoeae were demonstrated by indirect immunofluorescence (binding antibody) and by their ability to inhibit the attachment of gonococci to epithelial cells (functional antibody). Both IgG and IgA classes of immunoglobulin were involved, and the IgA component were primarily of a secreting (11S) nature. The ability of local genital antibody to inhibit attachment appears to persist for at least a short period of time and to be relatively strain specific.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avrameas S., Ternynck T. Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry. 1971 Dec;8(12):1175–1179. doi: 10.1016/0019-2791(71)90395-8. [DOI] [PubMed] [Google Scholar]
  2. Buchanan T. M. Antigenic heterogeneity of gonococcal pili. J Exp Med. 1975 Jun 1;141(6):1470–1475. doi: 10.1084/jem.141.6.1470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Buchanan T. M., Pearce W. A. Pili as a mediator of the attachment of gonococci to human erythrocytes. Infect Immun. 1976 May;13(5):1483–1489. doi: 10.1128/iai.13.5.1483-1489.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chipperfield E. J., Evans B. A. Effect of local infection and oral contraception on immunoglobulin levels in cervical mucus. Infect Immun. 1975 Feb;11(2):215–221. doi: 10.1128/iai.11.2.215-221.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Holmes K. K., Johnson D. W., Trostle H. J. An estimate of the risk of men acquiring gonorrhea by sexual contact with infected females. Am J Epidemiol. 1970 Feb;91(2):170–174. doi: 10.1093/oxfordjournals.aje.a121125. [DOI] [PubMed] [Google Scholar]
  6. James-Holmquest A. N., Swanson J., Buchanan T. M., Wende R. D., Williams R. P. Differential attachment by piliated and nonpiliated Neisseria gonorrhoeae to human sperm. Infect Immun. 1974 May;9(5):897–902. doi: 10.1128/iai.9.5.897-902.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KELLOGG D. S., Jr, PEACOCK W. L., Jr, DEACON W. E., BROWN L., PIRKLE D. I. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION. J Bacteriol. 1963 Jun;85:1274–1279. doi: 10.1128/jb.85.6.1274-1279.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kearns D. H., O'Reilly R. J., Lee L., Welch B. G. Secretory IgA antibodies in the urethral exudate of men with uncomplicated urethritis due to Neisseria gonorrhoeae. J Infect Dis. 1973 Jan;127(1):99–101. doi: 10.1093/infdis/127.1.99. [DOI] [PubMed] [Google Scholar]
  9. Kellogg D. S., Jr, Cohen I. R., Norins L. C., Schroeter A. L., Reising G. Neisseria gonorrhoeae. II. Colonial variation and pathogenicity during 35 months in vitro. J Bacteriol. 1968 Sep;96(3):596–605. doi: 10.1128/jb.96.3.596-605.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Koransky J. R., Scales R. W., Kraus S. J. Bacterial hemagglutination by Neisseria gonorrhoeae. Infect Immun. 1975 Sep;12(3):495–498. doi: 10.1128/iai.12.3.495-498.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  12. Novotny P., Turner W. H. Immunological heterogeneity of pili of Neisseria gonorrhoeae. J Gen Microbiol. 1975 Jul;89(1):87–92. doi: 10.1099/00221287-89-1-87. [DOI] [PubMed] [Google Scholar]
  13. O'Reilly R. J., Lee L., Welch B. G. Secretory IgA antibody responses to Neisseria gonorrhoeae in the genital secretions of infected females. J Infect Dis. 1976 Feb;133(2):113–125. doi: 10.1093/infdis/133.2.113. [DOI] [PubMed] [Google Scholar]
  14. Plaut A. G., Gilbert J. V., Artenstein M. S., Capra J. D. Neisseria gonorrhoeae and neisseria meningitidis: extracellular enzyme cleaves human immunoglobulin A. Science. 1975 Dec 12;190(4219):1103–1105. doi: 10.1126/science.810892. [DOI] [PubMed] [Google Scholar]
  15. Punsalang A. P., Jr, Sawyer W. D. Role of pili in the virulence of Neisseria gonorrhoeae. Infect Immun. 1973 Aug;8(2):255–263. doi: 10.1128/iai.8.2.255-263.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Reynolds H. Y., Thompson R. E. Pulmonary host defenses. I. Analysis of protein and lipids in bronchial secretions and antibody responses after vaccination with pseudomonas aeruginosa. J Immunol. 1973 Aug;111(2):358–368. [PubMed] [Google Scholar]
  17. Smith T. W., Haber E., Yeatman L., Butler V. P., Jr Reversal of advanced digoxin intoxication with Fab fragments of digoxin-specific antibodies. N Engl J Med. 1976 Apr 8;294(15):797–800. doi: 10.1056/NEJM197604082941501. [DOI] [PubMed] [Google Scholar]
  18. Steele E. J., Chaicumpa W., Rowley D. Further evidence for cross-linking as a protective factor in experimental cholera: properties of antibody fragments. J Infect Dis. 1975 Aug;132(2):175–180. doi: 10.1093/infdis/132.2.175. [DOI] [PubMed] [Google Scholar]
  19. Steele E. J., Chaicumpa W., Rowley D. Isolation and biological properties of three classes of rabbit antibody to Vibrio cholerae. J Infect Dis. 1974 Aug;130(2):93–103. doi: 10.1093/infdis/130.2.93. [DOI] [PubMed] [Google Scholar]
  20. Swanson J., Kraus S. J., Gotschlich E. C. Studies on gonococcus infection. I. Pili and zones of adhesion: their relation to gonococcal growth patterns. J Exp Med. 1971 Oct 1;134(4):886–906. doi: 10.1084/jem.134.4.886. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Swanson J., Sparks E., Zeligs B., Siam M. A., Parrott C. Studies on gonococcus infection. V. Observations on in vitro interactions of gonococci and human neutrophils. Infect Immun. 1974 Sep;10(3):633–644. doi: 10.1128/iai.10.3.633-644.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Swanson J. Studies on gonococcus infection. IV. Pili: their role in attachment of gonococci to tissue culture cells. J Exp Med. 1973 Mar 1;137(3):571–589. doi: 10.1084/jem.137.3.571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Thongthai C., Sawyer W. D. Studies on the virulence of Neisseria gonorrhoeae. I. Relation of colonial morphology and resistance to phagocytosis by polymorphonuclear leukocytes. Infect Immun. 1973 Mar;7(3):373–379. doi: 10.1128/iai.7.3.373-379.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tomasi T. B., Jr, Bienenstock J. Secretory immunoglobulins. Adv Immunol. 1968;9:1–96. doi: 10.1016/s0065-2776(08)60441-1. [DOI] [PubMed] [Google Scholar]
  25. Tramont E. C., Sadoff J. C., Artenstein M. S. Cross-reactivity of Neisseria gonorrhoeae and Neisseria meningitidis and the nature of antigens involved in the bactericidal reaction. J Infect Dis. 1974 Sep;130(3):240–247. doi: 10.1093/infdis/130.3.240. [DOI] [PubMed] [Google Scholar]
  26. Tramont E. C. Specificity of inhibition of epithelial cell adhesion of Neisseria gonorrhoeae. Infect Immun. 1976 Aug;14(2):593–595. doi: 10.1128/iai.14.2.593-595.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Waldman R. H., Cruz J. M., Rowe D. S. Immunoglobulin levels and antibody to Candida albicans in human cervicovaginal secretions. Clin Exp Immunol. 1972 Mar;10(3):427–434. [PMC free article] [PubMed] [Google Scholar]
  28. Waldman R. H., Ganguly R. Immunity to infections on secretory surfaces. J Infect Dis. 1974 Oct;130(4):419–440. doi: 10.1093/infdis/130.4.419. [DOI] [PubMed] [Google Scholar]
  29. Welch B. G., O'Reilly R. J. An indirect fluorescent-antibody technique for study of uncomplicated gonorrhea. I. Methodology. J Infect Dis. 1973 Jan;127(1):69–76. doi: 10.1093/infdis/127.1.69. [DOI] [PubMed] [Google Scholar]
  30. Williams R. C., Gibbons R. J. Inhibition of bacterial adherence by secretory immunoglobulin A: a mechanism of antigen disposal. Science. 1972 Aug 25;177(4050):697–699. doi: 10.1126/science.177.4050.697. [DOI] [PubMed] [Google Scholar]

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