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. 1990 Feb 11;18(3):493–499. doi: 10.1093/nar/18.3.493

The synthesis of polyamide-oligonucleotide conjugate molecules.

J Haralambidis 1, L Duncan 1, K Angus 1, G W Tregear 1
PMCID: PMC333453  PMID: 2308843

Abstract

We have developed methods for the synthesis of peptide-oligodeoxyribonucleotide conjugate molecules in particular, and polyamide-oligonucleotide conjugates in general. Synthesis is carried out by a solid-phase procedure and involves the assembly of a polyamide on the solid support, conversion of the terminal amino group to a protected primary aliphatic hydroxy group by reaction with alpha, omega-hydroxycarboxylic acid derivatives, and finally oligonucleotide synthesis using phosphoramidite chemistry. The conjugate molecules can be used as DNA probes, with the polyamide component carrying one or more non-radioactive markers. These conjugates also have the potential to be used as anti-sense inhibitors of gene expression, with the peptide segment acting as a targeting moiety.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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