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. 1990 Feb 11;18(3):625–629. doi: 10.1093/nar/18.3.625

Detection of a novel minisatellite-specific DNA-binding protein.

A Collick 1, A J Jeffreys 1
PMCID: PMC333471  PMID: 2308848

Abstract

We describe the detection of a ubiquitous DNA-binding protein which appears to interact specifically with tandem-repeated minisatellites. The murine 40 kd protein, which we term Msbp-1, was found to be present in all mouse tissues tested. This protein was bound specifically and with high affinity by double-stranded DNA containing a repeat sequence related to the minisatellite 'core' sequence, and binding required the presence of multiple repeat units. Corresponding minisatellite-specific DNA-binding proteins could also be detected in species ranging from Drosophila to man. This analysis represents the first direct evidence that minisatellites can function as a specific recognition signal for an endogenous DNA-binding protein.

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Selected References

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