Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1991 Feb 11;19(3):599–603. doi: 10.1093/nar/19.3.599

Genomic organization of lignin peroxidase genes of Phanerochaete chrysosporium.

J Gaskell 1, E Dieperink 1, D Cullen 1
PMCID: PMC333654  PMID: 2011531

Abstract

Three lignin peroxidase (LiP) genes from the basidiomycete Phanerochaete chrysosporium were cloned on a single 30 kb cosmid insert. One gene, GLG5, is the genomic equivalent of a previously reported cDNA clone, CLG5. The other two LiP genes are transcriptionally convergent and map to a position approximately 15 kb downstream of GLG5. The translational stop codons of these genes are separated by 1.3 kb. Analysis of homokaryons established allelic relationships to previously described LiP clones. Using clamped homogeneous electrical field electrophoresis (CHEF), seven chromosomal bands were resolved from P. chrysosporium genomic DNA. On CHEF gel Southern blots, the LiP gene family was localized to a single, dimorphic chromosome.

Full text

PDF
599

Images in this article

600Image
on p.600
601Image
on p.601
601Image
on p.601
601Image
on p.601

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alic M., Gold M. H. Genetic Recombination in the Lignin-Degrading Basidiomycete Phanerochaete chrysosporium. Appl Environ Microbiol. 1985 Jul;50(1):27–30. doi: 10.1128/aem.50.1.27-30.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anand R., Villasante A., Tyler-Smith C. Construction of yeast artificial chromosome libraries with large inserts using fractionation by pulsed-field gel electrophoresis. Nucleic Acids Res. 1989 May 11;17(9):3425–3433. doi: 10.1093/nar/17.9.3425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Andrawis A., Pease E. A., Kuan I. C., Holzbaur E., Tien M. Characterization of two lignin peroxidase clones from Phanerochaete chrysosporium. Biochem Biophys Res Commun. 1989 Jul 31;162(2):673–680. doi: 10.1016/0006-291x(89)92363-2. [DOI] [PubMed] [Google Scholar]
  4. Brody H., Carbon J. Electrophoretic karyotype of Aspergillus nidulans. Proc Natl Acad Sci U S A. 1989 Aug;86(16):6260–6263. doi: 10.1073/pnas.86.16.6260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brown A., Sims P. F., Raeder U., Broda P. Multiple ligninase-related genes from Phanerochaete chrysosporium. Gene. 1988 Dec 15;73(1):77–85. doi: 10.1016/0378-1119(88)90314-9. [DOI] [PubMed] [Google Scholar]
  6. Chu G., Vollrath D., Davis R. W. Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science. 1986 Dec 19;234(4783):1582–1585. doi: 10.1126/science.3538420. [DOI] [PubMed] [Google Scholar]
  7. Church G. M., Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991–1995. doi: 10.1073/pnas.81.7.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Corcoran L. M., Thompson J. K., Walliker D., Kemp D. J. Homologous recombination within subtelomeric repeat sequences generates chromosome size polymorphisms in P. falciparum. Cell. 1988 Jun 3;53(5):807–813. doi: 10.1016/0092-8674(88)90097-9. [DOI] [PubMed] [Google Scholar]
  9. Huoponen K., Ollikka P., Kälin M., Walther I., Mäntsälä P., Reiser J. Characterization of lignin peroxidase-encoding genes from lignin-degrading basidiomycetes. Gene. 1990 Apr 30;89(1):145–150. doi: 10.1016/0378-1119(90)90218-g. [DOI] [PubMed] [Google Scholar]
  10. Jeffries T. W., Choi S., Kirk T. K. Nutritional Regulation of Lignin Degradation by Phanerochaete chrysosporium. Appl Environ Microbiol. 1981 Aug;42(2):290–296. doi: 10.1128/aem.42.2.290-296.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kersten P. J. Glyoxal oxidase of Phanerochaete chrysosporium: its characterization and activation by lignin peroxidase. Proc Natl Acad Sci U S A. 1990 Apr;87(8):2936–2940. doi: 10.1073/pnas.87.8.2936. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kinscherf T. G., Leong S. A. Molecular analysis of the karyotype of Ustilago maydis. Chromosoma. 1988;96(6):427–433. doi: 10.1007/BF00303036. [DOI] [PubMed] [Google Scholar]
  13. Kirk T. K., Farrell R. L. Enzymatic "combustion": the microbial degradation of lignin. Annu Rev Microbiol. 1987;41:465–505. doi: 10.1146/annurev.mi.41.100187.002341. [DOI] [PubMed] [Google Scholar]
  14. Leisola M. S., Kozulic B., Meussdoerffer F., Fiechter A. Homology among multiple extracellular peroxidases from Phanerochaete chrysosporium. J Biol Chem. 1987 Jan 5;262(1):419–424. [PubMed] [Google Scholar]
  15. Ono B., Ishino-Arao Y. Inheritance of chromosome length polymorphisms in Saccharomyces cerevisiae. Curr Genet. 1988 Nov;14(5):413–418. doi: 10.1007/BF00521262. [DOI] [PubMed] [Google Scholar]
  16. Pease E. A., Andrawis A., Tien M. Manganese-dependent peroxidase from Phanerochaete chrysosporium. Primary structure deduced from cDNA sequence. J Biol Chem. 1989 Aug 15;264(23):13531–13535. [PubMed] [Google Scholar]
  17. Pribnow D., Mayfield M. B., Nipper V. J., Brown J. A., Gold M. H. Characterization of a cDNA encoding a manganese peroxidase, from the lignin-degrading basidiomycete Phanerochaete chrysosporium. J Biol Chem. 1989 Mar 25;264(9):5036–5040. [PubMed] [Google Scholar]
  18. Raeder U., Thompson W., Broda P. Genetic factors influencing lignin peroxidase activity in Phanerochaete chrysosporium ME446. Mol Microbiol. 1989 Jul;3(7):919–924. doi: 10.1111/j.1365-2958.1989.tb00241.x. [DOI] [PubMed] [Google Scholar]
  19. Raeder U., Thompson W., Broda P. RFLP-based genetic map of Phanerochaete chrysosporium ME446: lignin peroxidase genes occur in clusters. Mol Microbiol. 1989 Jul;3(7):911–918. doi: 10.1111/j.1365-2958.1989.tb00240.x. [DOI] [PubMed] [Google Scholar]
  20. Rustchenko-Bulgac E. P., Sherman F., Hicks J. B. Chromosomal rearrangements associated with morphological mutants provide a means for genetic variation of Candida albicans. J Bacteriol. 1990 Mar;172(3):1276–1283. doi: 10.1128/jb.172.3.1276-1283.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schalch H., Gaskell J., Smith T. L., Cullen D. Molecular cloning and sequences of lignin peroxidase genes of Phanerochaete chrysosporium. Mol Cell Biol. 1989 Jun;9(6):2743–2747. doi: 10.1128/mcb.9.6.2743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Smith T. L., Schalch H., Gaskell J., Covert S., Cullen D. Nucleotide sequence of a ligninase gene from Phanerochaete chrysosporium. Nucleic Acids Res. 1988 Feb 11;16(3):1219–1219. doi: 10.1093/nar/16.3.1219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Specht C. A., DiRusso C. C., Novotny C. P., Ullrich R. C. A method for extracting high-molecular-weight deoxyribonucleic acid from fungi. Anal Biochem. 1982 Jan 1;119(1):158–163. doi: 10.1016/0003-2697(82)90680-7. [DOI] [PubMed] [Google Scholar]
  25. Taub F., Thompson E. B. An improved method for preparing large arrays of bacterial colonies containing plasmids for hybridization: in situ purification and stable binding of DNA on paper filters. Anal Biochem. 1982 Oct;126(1):222–230. doi: 10.1016/0003-2697(82)90133-6. [DOI] [PubMed] [Google Scholar]
  26. Tien M., Tu C. P. Cloning and sequencing of a cDNA for a ligninase from Phanerochaete chrysosporium. Nature. 1987 Apr 2;326(6112):520–523. doi: 10.1038/326520a0. [DOI] [PubMed] [Google Scholar]
  27. Vollmer S. J., Yanofsky C. Efficient cloning of genes of Neurospora crassa. Proc Natl Acad Sci U S A. 1986 Jul;83(13):4869–4873. doi: 10.1073/pnas.83.13.4869. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wahl G. M., Lewis K. A., Ruiz J. C., Rothenberg B., Zhao J., Evans G. A. Cosmid vectors for rapid genomic walking, restriction mapping, and gene transfer. Proc Natl Acad Sci U S A. 1987 Apr;84(8):2160–2164. doi: 10.1073/pnas.84.8.2160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wilbur W. J., Lipman D. J. Rapid similarity searches of nucleic acid and protein data banks. Proc Natl Acad Sci U S A. 1983 Feb;80(3):726–730. doi: 10.1073/pnas.80.3.726. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. de Boer H. A., Zhang Y. Z., Collins C., Reddy C. A. Analysis of nucleotide sequences of two ligninase cDNAs from a white-rot filamentous fungus, Phanerochaete chrysosporium. Gene. 1987;60(1):93–102. doi: 10.1016/0378-1119(87)90217-4. [DOI] [PubMed] [Google Scholar]
  31. van de Pol T. J., Cremers F. P., Brohet R. M., Wieringa B., Ropers H. H. Derivation of clones from the choroideremia locus by preparative field inversion gel electrophoresis. Nucleic Acids Res. 1990 Feb 25;18(4):725–731. doi: 10.1093/nar/18.4.725. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES