Table I.
Structure and respective abbreviations used for disaccharides and hexasaccharides obtained from six different digestions
| Structural description | Abbreviationa | |
|---|---|---|
| Disaccharides | ΔUA(β1 → 3)GalNAc | ΔC0S |
| ΔUA(β1 → 3)GalNAc4S | ΔC4S | |
| ΔUA(β1 → 3)GalNAc6S | ΔC6S | |
| ΔUA2S(β1 → 3)GalNAc6S | ΔC2,6S | |
| Hexasaccharides | ΔUA(β1 → 3)GalNAc6S(β1 → 4)GlcA(β1 → 3)GalNAc6S(β1 → 4)GlcA(β1 → 3)GalNAc4S-ol | ΔC6;6;4S-ol |
| ΔUA(β1 → 3)GalNAc6S(β1 → 4)GlcA(β1 → 3)GalNAc4S(β1 → 4)GlcA(β1 → 3)GalNAc4S-ol | ΔC6;4;4S-ol | |
| ΔUA(β1 → 3)GalNAc4S(β1 → 4)GlcA(β1 → 3)GalNAc6S(β1 → 4)GlcA(β1 → 3)GalNAc4S-ol | ΔC4;6;4S-ol | |
| ΔUA(β1 → 3)GalNAc4S(β1 → 4)GlcA(β1 → 3)GalNAc4S(β1 → 4)GlcA(β1 → 3)GalNAc4S-ol | ΔC4;4;4S-ol | |
| ΔUA2S(β1 → 3)GalNAc6S(β1 → 4)GlcA(β1 → 3)GalNAc(β1 → 4)GlcA(β1 → 3)GalNAc6S | ΔC2,6;0;6S | |
| ΔUA2S(β1 → 3)GalNAc6S(β1 → 4)GlcA(β1 → 3)GalNAc(β1 → 4)GlcA(β1 → 3)GalNAc4S | ΔC2,6;0;4S | |
| ΔUA(β1 → 3)GalNAc6S(β1 → 4)GlcA(β1 → 3)GalNAc6S(β1 → 4)GlcA(β1 → 3)GalNAc6S-ol | ΔC6;6;6S-ol | |
| ΔUA(β1 → 3)GalNAc4S(β1 → 4)GlcA(β1 → 3)GalNAc6S(β1 → 4)GlcA(β1 → 3)GalNAc6S-ol | ΔC4;6;6S-ol | |
| ΔUA(β1 → 3)GalNAc4S(β1 → 4)GlcA2S(β1 → 3)GalNAc6S(β1 → 4)GlcA(β1 → 3)GalNAc6S-ol | ΔC4;2,6;6S-ol | |
| GlcA(β1 → 3)GalNAc6S(β1 → 4)GlcA(β1 → 3)GalNAc6S(β1 → 4)GlcA(β1 → 3)GalNAc-ol | C6;6;0S-ol | |
| GlcA(β1 → 3)GalNAc6S(β1 → 4)GlcA(β1 → 3)GalNAc4S(β1 → 4)GlcA(β1 → 3)GalNAc-ol | C6;4;0S-ol | |
| GlcA(β1 → 3)GalNAc6S(β1 → 4)GlcA(β1 → 3)GalNAc4S(β1 → 4)GlcA(β1 → 3)GalNAc4S-ol | C6;4;4S-ol | |
| GlcA(β1 → 3)GalNAc4S(β1 → 4)GlcA(β1 → 3)GalNAc4S(β1 → 4)GlcA(β1 → 3)GalNAc4S-ol | C4;4;4S-ol | |
| GlcA(β1 → 3)GalNAc6S(β1 → 4)GlcA(β1 → 3)GalNAc6S(β1 → 4)GlcA(β1 → 3)GalNAc4S-ol | C6;6;4S-ol | |
| GlcA(β1 → 3)GalNAc6S(β1 → 4)GlcA(β1 → 3)GalNAc6S(β1 → 4)GlcA2S(β1 → 3)GalNAc6S-ol | C6;6;2,6S-ol | |
| GlcA(β1 → 3)GalNAc6S(β1 → 4)GlcA2S(β1 → 3)GalNAc4S6S(β1 → 4)GlcA(β1 → 3)GalNAc6S-ol | C6;2,6;6S-ol | |
| GlcA2S(β1 → 3)GalNAc6S(β1 → 4)GlcA(β1 → 3)GalNAc4S(β1 → 4)GlcA2S(β1 → 3)GalNAc6S-ol | C2,6;4;2,6S-ol |
Nomenclature: ΔUA, Δ4,5unsaturated uronic acid; GalNAc, N-acetylgalactosamine; GlcA, glucuronic acid; S, sulfation group, where the numbers before “S” represent the ring position; -ol stands for reduced sugars (open rings at the reducing-ends).
aIn the structural codes, the comma (,) was used to separate hexoses, whereas semicolon (;) was used to separate disaccharide units. The digits 0, 4 and 6 denote respective positions of sulfation in the GalNAc units, whereas the 2 denotes 2-sulfation at uronic acid units.