Table II.
Yields of the disaccharides and the major hexasaccharides obtained from different digestion types within different time courses
| Digestion |
Disaccharides |
Hexasaccharides |
|||
|---|---|---|---|---|---|
| Type | Time | Absolute yield (%)a | Structure and relative yieldb | Absolute yield (%)a | Structure and relative yieldb |
| Near-complete digested btCS-A | 7 days | ∼80 | ΔC0S (8%); ΔC4S (50%); ΔC6S (42%) | <1 | Not determined |
| Near-complete digested scCS-C | 7 days | ∼85 | ΔC0S (3%); ΔC4S (26%); ΔC6S (49%); ΔC2,6S (22%) | <1 | Not determined |
| ABC lyase + btCS-A | 2 h 30 min | ∼70 | ΔC0S (7%); ΔC4S (60%); ΔC6S (33%) | ∼3 | ΔC6;6;4S-ol (20%); ΔC6;4;4S-ol or ΔC4;6;4S-ol (35%); ΔC4;4;4S-ol (46%) |
| ABC lyase + scCS-C | 2 h 30 min | ∼ 70 | ΔC0S (6%); ΔC4S (37%); ΔC6S (41%); ΔC2,6S (16%) | ∼3 | ΔC2,6;0;4S (34%); ΔC2,6;0;6S (33%) |
| C lyase + btCS-A | 2 days | ∼8 | ΔC0S (12%); ΔC4S (43%); ΔC6S (45%) | ∼10 | ΔC6;6;6S-ol (8%); ΔC4;6;6S-ol (20%); ΔC4;4;4S-ol (57%) |
| C lyase + scCS-C | 2 days | ∼8 | ΔC0S (14%); ΔC4S (15%); ΔC6S (68%); ΔC2,6S (3%) | ∼10 | ΔC6;6;6S-ol (22%); ΔC4;2,6;6S-ol (39%) |
| Hyaluronidase + btCS-A | 2 days | <1 | Not determined | ∼15 | C6;6;0S-ol (10%); C6;4;0S-ol (8%); C6;4;4S-ol (30%); C4;4;4S-ol (19%); C6;6;4S-ol (32%) |
| Hyaluronidase + scCS-C | 2 days | <1 | Not determined | ∼10 | C6;6;4S-ol (23%); C6;4;4S-ol (18%); C6;6;2,6S-ol (20%); C6;2,6;6S-ol (19%); C2,6;4;2,6S-ol (7%) |
aThe absolute yield was estimated as the percentage of weight (mg of sample) recovered from the peaks 2 (disaccharides) and 6 (hexasaccharides) from the SEC (Bio-Gel P-10 column) of each digestion type. The values are relative to ∼75 mg of loaded material into the column.
bThe relative yield was determined as the percentage of weight (mg of material) recovered as pure isomers fractionated by SAX-HPLC chromatography of the heterogeneous mixture of disaccharides or hexasaccharides from each digestion type. The values are compared with ∼5 mg of material loaded into the column.