Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1991 Feb 25;19(4):727–731. doi: 10.1093/nar/19.4.727

Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors.

D Hillemann 1, A Pühler 1, W Wohlleben 1
PMCID: PMC333703  PMID: 2017360

Abstract

For the isolation of single stranded plasmid DNA, various E. coli and E. coli-Streptomyces shuttle plasmids were equipped with the phage f1 replication origin. The transformation of some representative Streptomyces species with plasmid vectors occurred irrespective of whether single or double stranded DNA was used. In contrast, the transformation of Streptomyces was 10 to 100 times more efficient when an integration vector was in the single stranded form as opposed to the double stranded form. Streptomyces viridochromogenes was transformed by single stranded DNA integration vectors in order to replace the pat by the tsr gene and generate mutants unable to synthesize phosphinothricin-tripeptide (PTT).

Full text

PDF
727

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altenbuchner J., Cullum J. DNA amplification and an unstable arginine gene in Streptomyces lividans 66. Mol Gen Genet. 1984;195(1-2):134–138. doi: 10.1007/BF00332735. [DOI] [PubMed] [Google Scholar]
  2. Anzai H., Kumada Y., Hara O., Murakami T., Itoh R., Takano E., Imai S., Satoh A., Nagaoka K. Replacement of Streptomyces hygroscopicus genomic segments with in vitro altered DNA sequences. J Antibiot (Tokyo) 1988 Feb;41(2):226–233. doi: 10.7164/antibiotics.41.226. [DOI] [PubMed] [Google Scholar]
  3. Bayer E., Gugel K. H., Hägele K., Hagenmaier H., Jessipow S., König W. A., Zähner H. Stoffwechselprodukte von Mikroorganismen. 98. Phosphinothricin und Phosphinothricyl-Alanyl-Alanin. Helv Chim Acta. 1972 Jan 31;55(1):224–239. doi: 10.1002/hlca.19720550126. [DOI] [PubMed] [Google Scholar]
  4. Chater K. F., Bruton C. J. Mutational cloning in Streptomyces and the isolation of antibiotic production genes. Gene. 1983 Dec;26(1):67–78. doi: 10.1016/0378-1119(83)90037-9. [DOI] [PubMed] [Google Scholar]
  5. Gough J. A., Murray N. E. Sequence diversity among related genes for recognition of specific targets in DNA molecules. J Mol Biol. 1983 May 5;166(1):1–19. doi: 10.1016/s0022-2836(83)80047-3. [DOI] [PubMed] [Google Scholar]
  6. Harris J. E., Chater K. F., Bruton C. J., Piret J. M. The restriction mapping of c gene deletions in Streptomyces bacteriophage phi C31 and their use in cloning vector development. Gene. 1983 May-Jun;22(2-3):167–174. doi: 10.1016/0378-1119(83)90100-2. [DOI] [PubMed] [Google Scholar]
  7. Holmes D. S., Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981 Jun;114(1):193–197. doi: 10.1016/0003-2697(81)90473-5. [DOI] [PubMed] [Google Scholar]
  8. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T. Plasmid and phage vectors for gene cloning and analysis in Streptomyces. Methods Enzymol. 1987;153:116–166. doi: 10.1016/0076-6879(87)53052-x. [DOI] [PubMed] [Google Scholar]
  9. Kieser T. Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli. Plasmid. 1984 Jul;12(1):19–36. doi: 10.1016/0147-619x(84)90063-5. [DOI] [PubMed] [Google Scholar]
  10. Kieser T., Hopwood D. A., Wright H. M., Thompson C. J. pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors. Mol Gen Genet. 1982;185(2):223–228. doi: 10.1007/BF00330791. [DOI] [PubMed] [Google Scholar]
  11. Matsushima P., Cox K. L., Baltz R. H. Highly transformable mutants of Streptomyces fradiae defective in several restriction systems. Mol Gen Genet. 1987 Mar;206(3):393–400. doi: 10.1007/BF00428877. [DOI] [PubMed] [Google Scholar]
  12. Messing J., Vieira J. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982 Oct;19(3):269–276. doi: 10.1016/0378-1119(82)90016-6. [DOI] [PubMed] [Google Scholar]
  13. Norrander J., Kempe T., Messing J. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene. 1983 Dec;26(1):101–106. doi: 10.1016/0378-1119(83)90040-9. [DOI] [PubMed] [Google Scholar]
  14. Simon R., O'Connell M., Labes M., Pühler A. Plasmid vectors for the genetic analysis and manipulation of rhizobia and other gram-negative bacteria. Methods Enzymol. 1986;118:640–659. doi: 10.1016/0076-6879(86)18106-7. [DOI] [PubMed] [Google Scholar]
  15. Strauch E., Wohlleben W., Pühler A. Cloning of a phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tü494 and its expression in Streptomyces lividans and Escherichia coli. Gene. 1988;63(1):65–74. doi: 10.1016/0378-1119(88)90546-x. [DOI] [PubMed] [Google Scholar]
  16. Strauch E., Wohlleben W., Pühler A. Development of a plasmid-cloning system for Streptomyces viridochromogenes Tü494. J Basic Microbiol. 1987;27(8):449–455. doi: 10.1002/jobm.3620270812. [DOI] [PubMed] [Google Scholar]
  17. Thompson C. J., Ward J. M., Hopwood D. A. DNA cloning in Streptomyces: resistance genes from antibiotic-producing species. Nature. 1980 Jul 31;286(5772):525–527. doi: 10.1038/286525a0. [DOI] [PubMed] [Google Scholar]
  18. Tomich P. K. Streptomyces cloning: useful recombinant DNA systems and a summation of cloned genes. Antimicrob Agents Chemother. 1988 Oct;32(10):1465–1471. doi: 10.1128/aac.32.10.1465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Vieira J., Messing J. Production of single-stranded plasmid DNA. Methods Enzymol. 1987;153:3–11. doi: 10.1016/0076-6879(87)53044-0. [DOI] [PubMed] [Google Scholar]
  20. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  21. Wohlleben W., Arnold W., Broer I., Hillemann D., Strauch E., Pühler A. Nucleotide sequence of the phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tü494 and its expression in Nicotiana tabacum. Gene. 1988 Oct 15;70(1):25–37. doi: 10.1016/0378-1119(88)90101-1. [DOI] [PubMed] [Google Scholar]
  22. Wohlleben W., Pühler A. The Streptomyces ghanaensis low copy plasmid pSG2 and its use for vector construction. Arch Microbiol. 1987 Oct;148(4):298–304. doi: 10.1007/BF00456708. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES