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. 1991 Feb 25;19(4):809–813. doi: 10.1093/nar/19.4.809

A novel DNA joining activity catalyzed by T4 DNA ligase.

L M Western 1, S J Rose 1
PMCID: PMC333715  PMID: 2017363

Abstract

The use of T4 and E. coli DNA ligases in genetic engineering technology is usually associated with nick-closing activity in double stranded DNA or ligation of 'sticky-ends' to produce recombinant DNA molecules. We describe in this communication the ability of T4 DNA ligase to catalyze intramolecular loop formation between annealed oligodeoxyribonucleotides wherein Watson-Crick base pairing is absent on one side of the ligation site. Enzyme concentration, loop size, substrate specificity, and base composition were explored in an effort to maximize yield. Amounts of T4 DNA ligase in large molar excess to DNA template and ligated product are necessary to achieve high yields.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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