Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1991 Feb 25;19(4):929–932. doi: 10.1093/nar/19.4.929

A Musca domestica satellite sequence detects individual polymorphic regions in insect genome.

A Blanchetot 1
PMCID: PMC333733  PMID: 2017374

Abstract

A recombinant plasmid, pHMd.24, was constructed which contains an array of a 24 bp sequence from the genome of the Musca domestica. This sequence hybridizes to sites occurring at various genomic locations in different housefly strains. The pHMd.24 insert has a structural organisation similar to satellite DNA sequences and hybridizes to homologous repeated sequences representing a small fraction of the M. domestica genome. Interestingly, this clone reveals polymorphic fragments from DNA isolated from single houseflies, thus generating an insect DNA fingerprint. In addition, the segregation of the fragments detected by hybridisation in a M. domestica pedigree indicates a transmission that follows mendelian inheritance.

Full text

PDF
929

Images in this article

930Image
on p.930
931Image
on p.931
931Image
on p.931

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blanchetot A. Detection of highly polymorphic regions in insect genomes. Nucleic Acids Res. 1989 Apr 25;17(8):3313–3313. doi: 10.1093/nar/17.8.3313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brutlag D. L. Molecular arrangement and evolution of heterochromatic DNA. Annu Rev Genet. 1980;14:121–144. doi: 10.1146/annurev.ge.14.120180.001005. [DOI] [PubMed] [Google Scholar]
  3. Collick A., Jeffreys A. J. Detection of a novel minisatellite-specific DNA-binding protein. Nucleic Acids Res. 1990 Feb 11;18(3):625–629. doi: 10.1093/nar/18.3.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Crain W. R., Davidson E. H., Britten R. J. Contrasting patterns of DNA sequence arrangement in Apis mellifera (honeybee) and Musca domestica (housefly). Chromosoma. 1976 Dec 6;59(1):1–12. doi: 10.1007/BF00327705. [DOI] [PubMed] [Google Scholar]
  5. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  6. Gall J. G., Atherton D. D. Satellite DNA sequences in Drosophila virilis. J Mol Biol. 1974 Jan 5;85(4):633–664. doi: 10.1016/0022-2836(74)90321-0. [DOI] [PubMed] [Google Scholar]
  7. Georges M., Lequarré A. S., Castelli M., Hanset R., Vassart G. DNA fingerprinting in domestic animals using four different minisatellite probes. Cytogenet Cell Genet. 1988;47(3):127–131. doi: 10.1159/000132529. [DOI] [PubMed] [Google Scholar]
  8. Hough-Evans B. R., Jacobs-Lorena M., Cummings M. R., Britten R. J., Davidson E. H. Complexity of RNA in eggs of Drosophila melanogaster and Musca domestica. Genetics. 1980 May;95(1):81–94. doi: 10.1093/genetics/95.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jeffreys A. J., Wilson V., Thein S. L. Hypervariable 'minisatellite' regions in human DNA. Nature. 1985 Mar 7;314(6006):67–73. doi: 10.1038/314067a0. [DOI] [PubMed] [Google Scholar]
  10. John B., Miklos G. L. Functional aspects of satellite DNA and heterochromatin. Int Rev Cytol. 1979;58:1–114. doi: 10.1016/s0074-7696(08)61473-4. [DOI] [PubMed] [Google Scholar]
  11. Lohe A. R., Brutlag D. L. Identical satellite DNA sequences in sibling species of Drosophila. J Mol Biol. 1987 Mar 20;194(2):161–170. doi: 10.1016/0022-2836(87)90365-2. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES