Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1991 Feb 25;19(4):939–943. doi: 10.1093/nar/19.4.939

Selenium induces changes in the selenocysteine tRNA[Ser]Sec population in mammalian cells.

D Hatfield 1, B J Lee 1, L Hampton 1, A M Diamond 1
PMCID: PMC333735  PMID: 2017375

Abstract

Two isoacceptors of selenocysteine tRNA[Ser]Sec are present in higher vertebrates which are responsible for donating selenocysteine to protein. One such selenocysteine containing protein, glutathione peroxidase, requires selenium for its translation and transcription. Since tRNA[Ser]Sec is a critical component of the glutathione peroxidase translational machinery, the levels and distributions of its isoacceptors were examined from both human and rat cells grown in chemically defined media with and without selenium. Not only did the level of the selenocysteine tRNA[Ser]Sec population increase approximately 20% in cells grown in the presence of selenium, but the distributions of the two isoacceptors also changed relative to each other.

Full text

PDF
939

Images in this article

942Image
on p.942
942Image
on p.942

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chada S., Whitney C., Newburger P. E. Post-transcriptional regulation of glutathione peroxidase gene expression by selenium in the HL-60 human myeloid cell line. Blood. 1989 Nov 15;74(7):2535–2541. [PubMed] [Google Scholar]
  2. Chambers I., Frampton J., Goldfarb P., Affara N., McBain W., Harrison P. R. The structure of the mouse glutathione peroxidase gene: the selenocysteine in the active site is encoded by the 'termination' codon, TGA. EMBO J. 1986 Jun;5(6):1221–1227. doi: 10.1002/j.1460-2075.1986.tb04350.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  4. Diamond A. M., Montero-Puerner Y., Lee B. J., Hatfield D. Selenocysteine inserting tRNAs are likely generated by tRNA editing. Nucleic Acids Res. 1990 Nov 25;18(22):6727–6727. doi: 10.1093/nar/18.22.6727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Diamond A., Dudock B., Hatfield D. Structure and properties of a bovine liver UGA suppressor serine tRNA with a tryptophan anticodon. Cell. 1981 Aug;25(2):497–506. doi: 10.1016/0092-8674(81)90068-4. [DOI] [PubMed] [Google Scholar]
  6. Hatfield D., Diamond A., Dudock B. Opal suppressor serine tRNAs from bovine liver form phosphoseryl-tRNA. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6215–6219. doi: 10.1073/pnas.79.20.6215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hatfield D., Feng Y. X., Lee B. J., Rein A., Levin J. G., Oroszlan S. Chromatographic analysis of the aminoacyl-tRNAs which are required for translation of codons at and around the ribosomal frameshift sites of HIV, HTLV-1, and BLV. Virology. 1989 Dec;173(2):736–742. doi: 10.1016/0042-6822(89)90589-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hatfield D., Matthews C. R., Rice M. Aminoacyl-transfer RNA populations in mammalian cells chromatographic profiles and patterns of codon recognition. Biochim Biophys Acta. 1979 Oct 25;564(3):414–423. doi: 10.1016/0005-2787(79)90032-7. [DOI] [PubMed] [Google Scholar]
  9. Kelmers A. D., Heatherly D. E. Columns for rapid chromatographic separation of small amounts of tracer-labeled transfer ribonucleic acids. Anal Biochem. 1971 Dec;44(2):486–495. doi: 10.1016/0003-2697(71)90236-3. [DOI] [PubMed] [Google Scholar]
  10. Lee B. J., Rajagopalan M., Kim Y. S., You K. H., Jacobson K. B., Hatfield D. Selenocysteine tRNA[Ser]Sec gene is ubiquitous within the animal kingdom. Mol Cell Biol. 1990 May;10(5):1940–1949. doi: 10.1128/mcb.10.5.1940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lee B. J., Worland P. J., Davis J. N., Stadtman T. C., Hatfield D. L. Identification of a selenocysteyl-tRNA(Ser) in mammalian cells that recognizes the nonsense codon, UGA. J Biol Chem. 1989 Jun 15;264(17):9724–9727. [PubMed] [Google Scholar]
  12. Leinfelder W., Forchhammer K., Veprek B., Zehelein E., Böck A. In vitro synthesis of selenocysteinyl-tRNA(UCA) from seryl-tRNA(UCA): involvement and characterization of the selD gene product. Proc Natl Acad Sci U S A. 1990 Jan;87(2):543–547. doi: 10.1073/pnas.87.2.543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Leinfelder W., Stadtman T. C., Böck A. Occurrence in vivo of selenocysteyl-tRNA(SERUCA) in Escherichia coli. Effect of sel mutations. J Biol Chem. 1989 Jun 15;264(17):9720–9723. [PubMed] [Google Scholar]
  14. Mizutani T., Hashimoto A. Purification and properties of suppressor seryl-tRNA: ATP phosphotransferase from bovine liver. FEBS Lett. 1984 Apr 24;169(2):319–322. doi: 10.1016/0014-5793(84)80342-7. [DOI] [PubMed] [Google Scholar]
  15. Mullenbach G. T., Tabrizi A., Irvine B. D., Bell G. I., Tainer J. A., Hallewell R. A. Selenocysteine's mechanism of incorporation and evolution revealed in cDNAs of three glutathione peroxidases. Protein Eng. 1988 Sep;2(3):239–246. doi: 10.1093/protein/2.3.239. [DOI] [PubMed] [Google Scholar]
  16. O'Neill V. A., Eden F. C., Pratt K., Hatfield D. L. A human opal suppressor tRNA gene and pseudogene. J Biol Chem. 1985 Feb 25;260(4):2501–2508. [PubMed] [Google Scholar]
  17. Reddy A. P., Hsu B. L., Reddy P. S., Li N. Q., Thyagaraju K., Reddy C. C., Tam M. F., Tu C. P. Expression of glutathione peroxidase I gene in selenium-deficient rats. Nucleic Acids Res. 1988 Jun 24;16(12):5557–5568. doi: 10.1093/nar/16.12.5557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Takahashi K., Newburger P. E., Cohen H. J. Glutathione peroxidase protein. Absence in selenium deficiency states and correlation with enzymatic activity. J Clin Invest. 1986 Apr;77(4):1402–1404. doi: 10.1172/JCI112449. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES