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. 1991 Feb 25;19(4):945–948. doi: 10.1093/nar/19.4.945

Antiproliferative effects of antisense oligonucleotides directed to the RNA of c-myc oncogene.

G Degols 1, J P Leonetti 1, N Mechti 1, B Lebleu 1
PMCID: PMC333736  PMID: 1708128

Abstract

Several groups have reported the use of antisense oligonucleotides to inhibit c-myc gene expression and study its biological role. However high concentrations of free oligonucleotides were generally needed. To lower their concentration and stabilize the antisense effect against c-myc, oligonucleotides were covalently linked to poly(L-lysine) and administered in ternary complexes formed with heparin (100 micrograms/ml). A sequence specific growth inhibition was observed at concentrations lower than 1 microM, while oligonucleotide-poly(L-lysine) conjugates alone were inefficient. Similar results occurred with other polyanionic compounds. Inhibition of proliferation was correlated to a reduction of c-myc protein and to a transient decrease in c-myc mRNA level. However, implication of RNase H in this process could not be demonstrated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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