Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1991 Mar 11;19(5):1154. doi: 10.1093/nar/19.5.1154

Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products.

D Marchuk 1, M Drumm 1, A Saulino 1, F S Collins 1
PMCID: PMC333800  PMID: 2020552

Full text

PDF

Page 1154

1154

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aslanidis C., de Jong P. J. Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Res. 1990 Oct 25;18(20):6069–6074. doi: 10.1093/nar/18.20.6069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Clark J. M. Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases. Nucleic Acids Res. 1988 Oct 25;16(20):9677–9686. doi: 10.1093/nar/16.20.9677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hemsley A., Arnheim N., Toney M. D., Cortopassi G., Galas D. J. A simple method for site-directed mutagenesis using the polymerase chain reaction. Nucleic Acids Res. 1989 Aug 25;17(16):6545–6551. doi: 10.1093/nar/17.16.6545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jones D. H., Sakamoto K., Vorce R. L., Howard B. H. DNA mutagenesis and recombination. Nature. 1990 Apr 19;344(6268):793–794. doi: 10.1038/344793a0. [DOI] [PubMed] [Google Scholar]
  5. Kaufman D. L., Evans G. A. Restriction endonuclease cleavage at the termini of PCR products. Biotechniques. 1990 Sep;9(3):304–306. [PubMed] [Google Scholar]
  6. Mole S. E., Iggo R. D., Lane D. P. Using the polymerase chain reaction to modify expression plasmids for epitope mapping. Nucleic Acids Res. 1989 Apr 25;17(8):3319–3319. doi: 10.1093/nar/17.8.3319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Saiki R. K., Scharf S., Faloona F., Mullis K. B., Horn G. T., Erlich H. A., Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985 Dec 20;230(4732):1350–1354. doi: 10.1126/science.2999980. [DOI] [PubMed] [Google Scholar]
  8. Scharf S. J., Horn G. T., Erlich H. A. Direct cloning and sequence analysis of enzymatically amplified genomic sequences. Science. 1986 Sep 5;233(4768):1076–1078. doi: 10.1126/science.3461561. [DOI] [PubMed] [Google Scholar]
  9. Shuldiner A. R., Scott L. A., Roth J. PCR-induced (ligase-free) subcloning: a rapid reliable method to subclone polymerase chain reaction (PCR) products. Nucleic Acids Res. 1990 Apr 11;18(7):1920–1920. doi: 10.1093/nar/18.7.1920. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES