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. 1991 Mar 25;19(6):1213–1217. doi: 10.1093/nar/19.6.1213

CAAT/enhancer binding protein is able to bind to ATF/CRE elements.

O Bakker 1, M G Parker 1
PMCID: PMC333845  PMID: 2030941

Abstract

The transcription factor C/EBP is known to be able to bind to two different classes of sites, a CAAT-box and one present in a number of viral enhancers. In this paper we show using band-shift assays, methylation interference and footprinting that C/EBP is also able to bind with high affinity to ATF/CRE sites. Competition with mutant ATF sites and methylation interference indicate that C/EBP may be able to bind to the ATF/CRE sites by virtue of their homology to the enhancer core elements. Furthermore, we show that C/EBP is able to direct transcription from this site in transient transfection experiments and that mutations in the ATF binding site that impair DNA binding also effect the ability of C/EBP to stimulate transcription.

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