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. 1991 Mar 25;19(6):1291–1296. doi: 10.1093/nar/19.6.1291

Secondary pulsed field gel electrophoresis: a new method for faster separation of larger DNA molecules.

T Y Zhang 1, C L Smith 1, C R Cantor 1
PMCID: PMC333856  PMID: 2030945

Abstract

A novel technique, which we call secondary pulsed field gel electrophoresis (SPFG) has been developed. In SPFG, short pulses are applied in the direction of net migration of the DNA in addition to the reorienting pulses used in conventional pulsed field electrophoresis (PFG). Experimental results show that SPFG extends and improves the electrophoretic resolution of DNA for molecules from 0.5 megabase pairs to over 10 megabase pairs in size. This improved resolution is obtained with dramatically shorter run times. Thus SPFG appears to circumvent a number of the key limitations in previous PFG protocols.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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