Fig. 1.
Analysis of translational pausing using ribosome profiling in bacteria. a, Validation of the ribosome stalling site in the secM mRNA. b and c, Average ribosome occupancy of each codon relative to their respective tRNA abundance measured by Dong et al18. For growth in Luria broth (b), elevated occupancy at serine codons (blue) likely reflects preferential depletion of this amino acid. In glucose-rich medium (c), the ribosome occupancy is independent of tRNA abundance. d, Plot of cross-correlation function between ribosome occupancy profiles and the presence of the indicated tri-nucleotide sequences.