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. 1991 Apr 11;19(7):1399–1405. doi: 10.1093/nar/19.7.1399

The C-terminal domain of the Escherichia coli DNA gyrase A subunit is a DNA-binding protein.

R J Reece 1, A Maxwell 1
PMCID: PMC333892  PMID: 1851291

Abstract

We have constructed a clone which over-produces a 33 kDa protein representing the C-terminal portion of the Escherichia coli DNA gyrase A subunit. This protein has no enzymic activity of its own, but will form a complex with a 64 kDa protein (representing the N-terminal part of the A subunit) and the gyrase B subunit, that will efficiently catalyse DNA supercoiling. We show that the 33 kDa protein can bind to DNA on its own in a manner which induces positive supercoiling of the DNA. We propose that the 33 kDa protein represents a domain of the gyrase A subunit which is involved in the wrapping of DNA around DNA gyrase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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