Figure 3.

Chromatographic performance (e.g., RT reproducibility and peak width) is maintained during 1^st dimensional fractionation: mass chromatograms of ENL T43 peptide obtained under four fractionation conditions: (A) “simulated” 1D run using a single elution step (from 10.8 to 50% ACN); (B) fraction 2 out of 3 (from 10.8 to 18.6% ACN); (C) fraction 4 out of 5 (from 15.4 to 18.6% ACN); (D) fraction 5 out of 10 (from 15.4 to 16.7% ACN). The data was acquired in continuum mode and all mass chromatograms used an extraction window of 0.1 Da around the corresponding monoisotopic peak. All separations used a 30 min gradient (7–35% ACN, 0.1% FA).