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. 1991 Apr 11;19(7):1527–1532. doi: 10.1093/nar/19.7.1527

Oxalyl-CPG: a labile support for synthesis of sensitive oligonucleotide derivatives.

R H Alul 1, C N Singman 1, G R Zhang 1, R L Letsinger 1
PMCID: PMC333911  PMID: 2027761

Abstract

A procedure is described for linking nucleosides covalently to controlled pore glass or cross-linked polystyrene supports by means of an oxalyl anchor. Though stable to triethylamine and diisopropylamine, the nucleoside-oxalyl link can be cleaved within a few minutes at room temperature with ammonium hydroxide in methanol. This new anchor can be used in automated synthesis of conventional oligonucleotides. The primary value, however, is that it enables one to employ solid support methodology to synthesize a variety of base-sensitive oligonucleotide derivatives, as illustrated here by synthesis of oligomers with base protecting groups intact and with methyl phosphotriester groups at the internucleoside links.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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