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Nucleic Acids Research logoLink to Nucleic Acids Research
. 1991 Apr 11;19(7):1577–1583. doi: 10.1093/nar/19.7.1577

Characterization of HIV-1 REV protein: binding stoichiometry and minimal RNA substrate.

K S Cook 1, G J Fisk 1, J Hauber 1, N Usman 1, T J Daly 1, J R Rusche 1
PMCID: PMC333918  PMID: 2027765

Abstract

The HIV-1 REV protein binds to the stem II region of the REV-responsive element (RNA). Studies to further define the RNA sequence and structure specifically bound by REV protein identify a minimal RNA element of 40 nucleotides. Analysis of RNA fragments by gel retardation and filter binding suggest that a core element composed of one particular stem with flanking sequences capable of forming a second double stranded region is essential for specific recognition by REV protein. Stable REV-RNA complexes are formed in a stoichiometry of 1 REV: 1 RNA. The minimal RNA element binds 1 REV molecule while the stem II saturates at 3 REV molecules per RNA. These results establish that REV recognizes a primary binding site within the RRE and support the notion that the initial viral transcript binding event involves a monomeric REV protein.

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Selected References

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