Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1992 Jul 25;20(14):3773–3777. doi: 10.1093/nar/20.14.3773

Transfer RNA genes in the mitochondrial genome from a liverwort, Marchantia polymorpha: the absence of chloroplast-like tRNAs.

K Oda 1, K Yamato 1, E Ohta 1, Y Nakamura 1, M Takemura 1, N Nozato 1, K Akashi 1, K Ohyama 1
PMCID: PMC334030  PMID: 1641342

Abstract

Twenty-nine genes for 27 species of tRNAs were deduced from the complete nucleotide sequence of the mitochondrial genome from a liverwort, Marchantia polymorpha. One to three species of tRNA genes corresponded to each of 20 amino acids including three species for leucine and arginine, two species for serine and glycine, and one for the rest of the amino acids. Interestingly, all tRNA genes were located in the semicircle of the liverwort mitochondrial genome except for the trnY and trnR genes. The region containing these tRNA genes was originally duplicated, and two trnR genes have diverged from each other. On the other hand, trnY and trnfM are present as two identical copies. The G:U and U:N wobbling between the first nucleotide of the anticodon and the third nucleotide of the codon permit the 27 tRNA identified species to translate almost all codons. However, at least two additional tRNA genes, trnl-GAU for AUY codon and trnT-UGU for ACR codon, are required to read all codons used in the liverwort mitochondrial genome. All of the identified tRNA genes are 'native' in liverwort mitochondria, not 'chloroplast-like' tRNAs as are found in the mitochondria of higher plants. This result implies that the tRNA gene transfer from chloroplast to mitochondrial genome in higher plants has occurred after the divergence from bryophytes.

Full text

PDF
3773

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Binder S., Schuster W., Grienenberger J. M., Weil J. H., Brennicke A. Genes for tRNA(Gly), tRNA(His), tRNA(Lys), tRNA(Phe), tRNA(Ser) and tRNA(Tyr) are encoded in Oenothera mitochondrial DNA. Curr Genet. 1990 Apr;17(4):353–358. doi: 10.1007/BF00314884. [DOI] [PubMed] [Google Scholar]
  2. Gray M. W., Cedergren R., Abel Y., Sankoff D. On the evolutionary origin of the plant mitochondrion and its genome. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2267–2271. doi: 10.1073/pnas.86.7.2267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Izuchi S., Sugita M. Nucleotide sequence of a tomato mitochondrial tRNA(Cvs) (GCA) gene. Nucleic Acids Res. 1989 Feb 11;17(3):1248–1248. doi: 10.1093/nar/17.3.1248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Joyce P. B., Gray M. W. Chloroplast-like transfer RNA genes expressed in wheat mitochondria. Nucleic Acids Res. 1989 Jul 25;17(14):5461–5476. doi: 10.1093/nar/17.14.5461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Joyce P. B., Gray M. W. Nucleotide sequence of a wheat mitochondrial glutamine tRNA gene. Nucleic Acids Res. 1988 Feb 11;16(3):1210–1210. doi: 10.1093/nar/16.3.1210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lagerkvist U. "Two out of three": an alternative method for codon reading. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1759–1762. doi: 10.1073/pnas.75.4.1759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Maréchal-Drouard L., Guillemaut P., Cosset A., Arbogast M., Weber F., Weil J. H., Dietrich A. Transfer RNAs of potato (Solanum tuberosum) mitochondria have different genetic origins. Nucleic Acids Res. 1990 Jul 11;18(13):3689–3696. doi: 10.1093/nar/18.13.3689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Maréchal-Drouard L., Weil J. H., Guillemaut P. Import of several tRNAs from the cytoplasm into the mitochondria in bean Phaseolus vulgaris. Nucleic Acids Res. 1988 Jun 10;16(11):4777–4788. doi: 10.1093/nar/16.11.4777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Oda K., Kohchi T., Ohyama K. Mitochondrial DNA of Marchantia polymorpha as a single circular form with no incorporation of foreign DNA. Biosci Biotechnol Biochem. 1992 Jan;56(1):132–135. doi: 10.1271/bbb.56.132. [DOI] [PubMed] [Google Scholar]
  10. Oda K., Yamato K., Ohta E., Nakamura Y., Takemura M., Nozato N., Akashi K., Kanegae T., Ogura Y., Kohchi T. Gene organization deduced from the complete sequence of liverwort Marchantia polymorpha mitochondrial DNA. A primitive form of plant mitochondrial genome. J Mol Biol. 1992 Jan 5;223(1):1–7. doi: 10.1016/0022-2836(92)90708-r. [DOI] [PubMed] [Google Scholar]
  11. Ohyama K., Fukuzawa H., Kohchi T., Sano T., Sano S., Shirai H., Umesono K., Shiki Y., Takeuchi M., Chang Z. Structure and organization of Marchantia polymorpha chloroplast genome. I. Cloning and gene identification. J Mol Biol. 1988 Sep 20;203(2):281–298. doi: 10.1016/0022-2836(88)90001-0. [DOI] [PubMed] [Google Scholar]
  12. Ojala D., Montoya J., Attardi G. tRNA punctuation model of RNA processing in human mitochondria. Nature. 1981 Apr 9;290(5806):470–474. doi: 10.1038/290470a0. [DOI] [PubMed] [Google Scholar]
  13. Okimoto R., Wolstenholme D. R. A set of tRNAs that lack either the T psi C arm or the dihydrouridine arm: towards a minimal tRNA adaptor. EMBO J. 1990 Oct;9(10):3405–3411. doi: 10.1002/j.1460-2075.1990.tb07542.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pfitzinger H., Weil J. H., Pillay D. T., Guillemaut P. Codon recognition mechanisms in plant chloroplasts. Plant Mol Biol. 1990 May;14(5):805–814. doi: 10.1007/BF00016513. [DOI] [PubMed] [Google Scholar]
  15. Sangaré A., Lonsdale D., Weil J. H., Grienenberger J. M. Sequence analysis of the tRNA(Tyr) and tRNA(Lys) genes and evidence for the transcription of a chloroplast-like tRNA(Met) in maize mitochondria. Curr Genet. 1989 Sep;16(3):195–201. doi: 10.1007/BF00391477. [DOI] [PubMed] [Google Scholar]
  16. Sangaré A., Weil J. H., Grienenberger J. M., Fauron C., Lonsdale D. Localization and organization of tRNA genes on the mitochondrial genomes of fertile and male sterile lines of maize. Mol Gen Genet. 1990 Sep;223(2):224–232. doi: 10.1007/BF00265058. [DOI] [PubMed] [Google Scholar]
  17. Weber F., Dietrich A., Weil J. H., Maréchal-Drouard L. A potato mitochondrial isoleucine tRNA is coded for by a mitochondrial gene possessing a methionine anticodon. Nucleic Acids Res. 1990 Sep 11;18(17):5027–5030. doi: 10.1093/nar/18.17.5027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wintz H., Chen H. C., Pillay D. T. Nucleotide sequence of a soybean mitochondria tRNA Glu (TTC) gene. Nucleic Acids Res. 1987 Dec 23;15(24):10588–10588. doi: 10.1093/nar/15.24.10588. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. de Bruijn M. H., Schreier P. H., Eperon I. C., Barrell B. G., Chen E. Y., Armstrong P. W., Wong J. F., Roe B. A. A mammalian mitochondrial serine transfer RNA lacking the "dihydrouridine" loop and stem. Nucleic Acids Res. 1980 Nov 25;8(22):5213–5222. doi: 10.1093/nar/8.22.5213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. de Zamaroczy M., Bernardi G. The primary structure of the mitochondrial genome of Saccharomyces cerevisiae--a review. Gene. 1986;47(2-3):155–177. doi: 10.1016/0378-1119(86)90060-0. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES