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. 1992 Aug 11;20(15):3845–3850. doi: 10.1093/nar/20.15.3845

Structure of the human DNA ligase I gene.

P Noguiez 1, D E Barnes 1, H W Mohrenweiser 1, T Lindahl 1
PMCID: PMC334057  PMID: 1508669

Abstract

The gene encoding DNA ligase I, the major DNA ligase activity in proliferating mammalian cells, maps to human chromosome 19q13.2-13.3. We have determined the complete structure of the gene, which is composed of 28 exons spanning 53kb on this chromosome. The first exon is untranslated, and utilises a GC dinucleotide instead of the canonical GT splice donor. The 5' flanking region lacks a TATA box and is highly GC-rich, as is characteristic of a 'housekeeping' gene. In common with the promoters of genes encoding other DNA replication enzymes, such as DNA polymerase alpha, the 5' flanking region of the DNA ligase I gene contains recognition elements for several transcription factors which may mediate increased expression in quiescent cells in response to growth factors.

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Selected References

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