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. 1992 Aug 11;20(15):3983–3986. doi: 10.1093/nar/20.15.3983

Atomic force microscopy of reovirus dsRNA: a routine technique for length measurements.

Y L Lyubchenko 1, B L Jacobs 1, S M Lindsay 1
PMCID: PMC334076  PMID: 1508683

Abstract

Atomic force microscopy (AFM) was used to image reovirus double stranded RNA (dsRNA) deposited from diluted buffer solution onto a chemically treated mica surface. This procedure allows AFM images of dsRNA molecules to be obtained with a quality close to that obtained with conventional electron microscopy. The length of the molecules were measured directly on a computer display using the digitally acquired images. The lengths of the molecules varied between 0.2 and 1.8 microns. Statistical analysis showed a multimodal distribution with clear maxima at 0.4, 0.65 and 1.05 microns. These data are in a good agreement with those obtained by electron microscopy and gel electrophoresis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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