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. 1992 Aug 25;20(16):4179–4185. doi: 10.1093/nar/20.16.4179

Bleomycin mediated degradation of DNA-RNA hybrids does not involve C-1' chemistry.

M J Absalon 1, C R Krishnamoorthy 1, G McGall 1, J W Kozarich 1, J Stubbe 1
PMCID: PMC334123  PMID: 1380696

Abstract

Incubation of Fe(II) bleomycin and O2 with a number of 'A'-like DNA-RNA hybrid homopolymers at 4 atm O2 results in formation of base propenal and base in a ratio of approximately 1.0:1.0. This ratio differs dramatically from the corresponding ratio of approximately 10:1.0 observed when activated BLM degrades 'B'-like DNA homopolymers. Experiments were undertaken to determine if the shift to enhanced base production observed in the A-like hybrids is the result of C-1' chemistry in addition to the C-4' chemistry normally observed with B-like DNA under identical conditions. Increased accessibility of the 1'-hydrogen might be anticipated due to widening of the minor groove in the A-like conformers. Experiments using poly([1'-3H]dA) poly(rU) and poly([U-14C]dA) poly(rU) indicated that neither 3H2O nor deoxyribonolactone accompanied adenine release. In addition, studies using poly([4'-2H]dA) poly(rU) and poly([1'-2H]dA) poly(rU) unambiguously establish that the altered base to base propenal ratio is not the result of C-1' chemistry, but a direct consequence of C-4' chemistry.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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