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. 1992 Aug 25;20(16):4221–4227. doi: 10.1093/nar/20.16.4221

A ribosomal ambiguity mutation in the 530 loop of E. coli 16S rRNA.

M O'Connor 1, H U Göringer 1, A E Dahlberg 1
PMCID: PMC334129  PMID: 1380697

Abstract

A series of base substitution and deletion mutations were constructed in the highly conserved 530 stem and loop region of E. coli 16S rRNA involved in binding of tRNA to the ribosomal A site. Base substitution and deletion of G517 produced significant effects on cell growth rate and translational fidelity, permitting readthrough of UGA, UAG and UAA stop codons as well as stimulating +1 and -1 frameshifting in vivo. By contrast, mutations at position 534 had little or no effect on growth rate or translational fidelity. The results demonstrate the importance of G517 in maintaining translational fidelity but do not support a base pairing interaction between G517 and U534.

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Selected References

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