a, DNL measured in vivo in fed, 4-month-old male mice PG=perigonadal, SC=subcutaneous, (n=5–6 per group). b, Quantification of western blots of FAS and ACC in SC fat from fed, 6-month-old females (n=9–10 per group). For a and b, *P<0.05 versus same ChREBP genotype, different AG4OX genotype. # p<0.05 versus same AG4OX genotype, different ChREBP genotype. c, Body weights in male mice on chow (n=5–7 per group). *P<0.05 versus all other groups at the indicated time. d, Body composition in 8-week-old, female mice (for d–g; n=10–12 per group). *P<0.01 versus all others. e, Glucose tolerance test and f, insulin tolerance test. *P<0.05 versus all others; #P<0.05 versus WT and ChREBP KO. g, Glycemia following food removal * P<0.05 versus all others; # P<0.05 versus ChREBP KO and AG4OX-ChREBP KO; †P<0.05 versus AG4OX; Values are means ± S.E.